CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



437 



Variants: Authors added 2.0 or 4.0% NaCl 



to the basic agar solution. 

 Reference: Fred and Peterson (1922 p. 



261). 



1498. Stuart's Basal Asparagin Agar 

 Constituents: 



1. Agar solution 1000.0 cc. 



2. Asparagin 10.0 g. 



Preparation : 



(1) Prepare an agar solution using 

 washed agar prepared according to 

 medium 1398. 



(2) Dissolve one of the added nutrients in 

 (1). 



(3) Adjust the reaction between pH-7.0 

 and 7.2. 



Sterilization: Method not given. 



Use: To study variation in Bacterium 



typhosum. 

 Added nutrients: The author added one 



of the following: 



NaNOa 20g 



KNO3 2.0 g." 



NH4NO3 2.0 g. 



Bodium phosphate 2.0 g. 



potassium phosphate., 2.0 g. 



Variants : 



(a) The author used 4.0 g. asparagin and 

 added 2.0 g. sodium phosphate, 6.0 g. 

 ammonium lactate and 5.0 g. NaCl. 



(b) The author used 3.4 g. asparagin in 

 the basic agar solution and added 

 1.0 g. potassium phosphate, 10.0 g. 

 ammonium lactate 5.0 g. NaCl, 

 0.2 g. MgS04, 0.1 g. CaClj and 40.0 g. 

 glycerol. 



Reference: Stuart (1924 p. 586). 



1499. Long's Basal Glycerol Asparagin Agar 



(Uyei) 

 Constituents: 



1- Water 1000.0 cc. 



2- Agar 25.0 g 



3. Glycerol 5O.0 g 



4. Ferrous ammonium ci- 

 trate 0.05 g 



5. MgS04 10 g 



6. NaCl 2.0 cr 



7. Na.COa 3.0 



8. KH2PO4... 



9. Ammonium citrate. 

 10. Asparagin 



3.0 

 5.0 

 5.0 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8, 9 and 10 in 1. 



(2) Add one of the added nutrients. 

 Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. The 

 author reported that e.xtracts of tubercle 

 bacilli failed to show the presence of any 

 growth stimulating substances. Cod 

 liver oil concentrate e.xerted an indifferent 

 action, while concentrations of 1:1000 

 Harris yeast vitamin, 1:100 tomato juice 

 and orange juice, or 1:20 cabbage, exerted 

 an acceleration of growth. 

 Added nutrients: The author added one of 

 the following in various concentrations: 



(a) Cod liver oil concentrate (Oscodal of 

 Funk and Dubin). 



(b) Harris yeast vitamin. 



(c) Tomato juice. 



(d) Orange juice. 



(e) Cabbage. 



(f) Tubercle bacilli extracts. 

 Variants: The author used the basic 



medium without any additions. 

 Reference: Uyei (1927 p. 427), (1927 p. 

 437). 



1500. Noyes' Asparaginate Agar 

 Constituents: 



1. Water 1000.0 cc. 



2. Agar (best) 15.0 g. 



3. Sodium asparaginate 1.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 

 Sterilization: Method not given. 

 Use: General culture medium. Primarily 



used for study of soil organisms. 

 Reference: Noyes (1916 pp. 93, 94). 



1501. Sullivan's Salt Asparagin Agar 

 Constituents: 



1- Water looO.O cc. 



2. Asparagin IQ.O g. 



3. NaCl 10.0 g. 



4. MgS04 3.0 g. 



5. Agar lo.O g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization : Method not given. 

 Use: General synthetic culture medium. 



Also used to study pigment production. 



Author reported that most of the chromo- 



genic bacteria did not produce color on 



