438 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



this medium. B. pyocijaneus produced 

 abundant pigment, however. 

 Reference: Sullivan (1905-C6 p. 117). 



1502. Davis' Asparagin Phosphate Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. Asparagin,. 20.0 g. 



4. MgS04 10-0 g- 



5. K..>HP04 10-0 g- 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



Sterilization: Method not given. 



Use : To produce chlamydospores by Sporo- 

 thrix schenckii. Author reported that 

 the growth of the organism was slightly 

 accelerated if pure or impure sugars be 

 added. 



Reference: Davis (1914 p. 485), (1915 p. 

 179). 



1503. Bengis' Phosphate Asparagin Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Asparagin 10.0 g. 



3. Agar (powdered) 30.0 g. 



4. Na2HP04 2.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Filter into incubating flasks. 

 Sterilization: Sterilize in Bramhall-Dean 



autoclave. 

 Use: To cultivate large quantities of B. 



coli. 

 Variants : 



(a) The author used 2.0 g. (NH4)2HP04 

 instead of Na2HP04. 



(b) Tanner described a similar medium 

 as Dolt's asparagin agar. One of 

 these media was composed of 100.0 

 cc. of distilled water, 350.0 cc. of a 

 3.0% purified agar solution in water, 

 2.5 g. asparagin and 0.5 g. Na2HP04. 

 The other medium contained 250.0 

 cc. distilled water, 250.0 cc. of a 

 3.0% purified agar solution, 6.25 g. 

 asparagin and 1.25 g. Na2HP04. 

 Tanner reported that these media 

 would give a good growth of B. coli 

 in 24 hours. 



References: Bengis (1916 p. 392), Tanner 

 (1919 pp. 50, 65). 



1504. Conn's Glucose Asparaginate Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar 12.0 g. 



3. Sodium asparaginate 1-0 g. 



4. Glucose 1-0 g. 



5. MgS04 0.2 g. 



6. NH4H2PO4 1-5 g. 



7. CaClj 0.1 g. 



8. KCl 0.1 g. 



9. FeCl3 trace.. 



Preparation : 



(1) Dissolve 2, 5, 6, 7, 8 and 9 in 1. 



(2) Adjust to 0.8 or 1.0% normal acid to 

 phenolphthalein. 



(3) May be clarified with egg or by 

 simply heating for 30 minutes at 15 

 pounds pressure in such a way as not 

 to disturb the sediment and then 

 decanting thru cotton filter. 



(4) Add 1.0 g. glucose and 1.0 g. sodium 

 asparaginate to (3). 



Sterilization: Method not given. 



Use: Bacterial counts in soils. Author 

 reported that there may be other satis- 

 factory combinations of the salts than the 

 one given. The amount of glucose and 

 asparaginate were increased without 

 varying the count. 



References: Conn (1916 p. 722), Giltner 

 (1921 p. 372). 



1505. Kisch's Glucose Asparagin Agar 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 10 g. 



3. MgS04 0.5 g. 



4. NaCl 0.02 g. 



5. reS04 trace 



6. Calcium phosphate trace 



7. Dextrose 10.0 g. 



8. Asparagin 1-9 g- 



9. Agar (2.0%) 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add NasCOa until the reaction is 

 slightly alkaline to litmus. 



(3) Dissolve 9 in (2). 



(4) Dissolve 8 in (3). 

 Sterilization: Not specified. 



Use: To study nitrogen requirements for 



colon typhoid group. 

 Reference: Kisch (1918-19 p. 32). 



