CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



439 



1506. Dolt's Lactose Asparagin Agar 

 Constituents: 



1. Distilled water 1000.0 ec. 



2. Agar, purified 30.0 g. 



3. Asparagin 10.0 g. 



4. NasHPO^ 2.0 g. 



5. Lactose 10.0 g. 



6. Azolitmin (1.0% solution).. 10.0 cc. 

 Preparation : 



(1) Purify agar by cutting it in small 

 pieces and soaking it in distilled water 

 for 24 hours. 



(2) Dissolve (1), 2, 3 and 4 in 1. 



(3) Make neutral to phenolphthalein 

 with NaOH. 



(4) Add 5. 



(5) Prepare 6 by dissolving 1.0 g. of 

 Kahlbaum's C.P. azolitmin in 100.0 

 g. distilled water and boil for 15 

 minutes. It is a blue solution after 

 boiling. 



(6) Add (5) to (4). 

 Sterilization: Method not given. 



Use: Detection of acid forming bacteria in 

 water analysis. Author reported that 

 B. coli developed red colonies. 



Reference: Dolt (1908 p. 621). 



1507. Groenewege's Starch Asparagin Agar 

 Constituents: 



1. Water agar 1000.0 cc. 



2. Starch 10 g. 



3. Asparagin 1 -0 g 



4. K0HPO4 0.5 g^ 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1000.0 cc. tap 

 water agar. (Composition not 

 given.) 

 Sterilization: Method not given. 

 Use: Diastase production by Phytobacter 

 ly coper sicum n. sp. (causing tomato rot). 

 Author reported that when diastase was 

 produced, the starch disappeared. 

 Reference: Groenewege (1913 p. 28). 



1508. Noyes' Starch Asparaginate Agar 

 Constituents: 



1- Water 1000.0 cc. 



2. Agar (best) 15.0 g. 



3. Starch 2.0 g. 



4. Sodium asparaginate 1.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Method not specified. 



Use: General culture medium, primarily 



used to study organisms of the soil. 

 Reference: Noyes (1916 pp. 93-94). 



1509. Stutzer and Hartleb's Glycerol 

 Asparagin Agar 

 Constituents: 



1- Water 1000.0 cc. 



2. Agar 2O.O g. 



3. Asparagin 10 g. 



4. Glycerol 1.0 g. 



5. Potassium phosphate 1-0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, and 5 in 1. 

 Sterilization: Not specified. 

 Variants : Fraenkel used 15.0 g. agar instead 



of 20.0 g. 

 References: Stutzer and Hartleb (1897 p. 



403), Fraenkel (1898 p. 10). 



1510. Sullivan's Glycerol Asparagin Agar 

 Constituents : 



1. Water 1000.0 cc. 



2. Asparagin 10.0 g. 



3. Glycerol 35.0 g. 



4. K2HPO4 1.0 g. 



5. (NH4)3P04 10.0 g. 



6. MgS04 2.0 g. 



7. Agar 10. g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Medium is slightly acid due to evap- 

 oration of NH3 in sterilization. 



Sterilization : Method not given. 



Use: General culture medium. Author 



reported that B. typhosus did not grow in 



this medium. 

 Reference: Sullivan (1905-06 p. 119). 



1511. Peklo's Mannitol Asparagin Agar 

 Constituents : 



1. Distilled water 500.0 cc. 



2. KH2PO4 1.0 g- 



3. K2HPO4 1.0 g 



4. MgS04 0.6 g 



5. NaCl 0.2 g 



6. CaCl, 0.1 g 



7. Asparagin 2.0 g 



8. Mannitol 10.0 g, 



9. Agar 0.75% 



10. FeaCle trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5, and 6 in 200.0 cc. 

 of distilled water. 



