440 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(2) To 100.0 cc. of (1) add 400.0 cc. dis- 

 tilled water and dissolve 2.0 g. as- 

 paragin, 1.0 g. mannitol and a trace of 

 Fe2Cl6 in the mixture. 



(3) From sterile (2) prepare a 0.75% agar. 

 (Method or final sterilization not 

 given.) 



Sterilization: Sterilize (2) several times 

 (method not given). A turbidity forms 

 but disappears when the medium cools. 



Use: Cultivation of plant actinomyces. 

 Author reported good growth. 



Reference: Peklo (1910 p. 473). 



1512. Thornton's Mannitol Asparagin Agar 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 ,. 10 g. 



3. MgS04-7H20 0.2 g. 



4. CaCU 0.1 g. 



5. FeCls 0.002 g. 



6. KNO3. 0.5 g. 



7. Asparagin 0.5 g. 



8. NaCl 0.1 g. 



9. Agar., 15.0 g. 



10. Mannitol 10 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1 by 

 steaming for 30 minutes. 



(2) Neutralize to turmeric paper. 



(3) Filter thru cotton-wool. 



(4) Add 1.0 g. mannitol to (3). 



(5) Tube in 8.0 cc. quantities. 

 Sterilization: Sterilize in the autoclave at 



22.5 pounds pressure. 

 Use: Cultivation of soil forms. 

 References: Thornton (1922 p. 241) taken 



from (1923 p. 277), Cunningham (1924 



p. 136). 



1513. Conn's Glycerol Asparaginate Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. Glvcerol 10.0 cc. 



4. K2HPO4 0.5 g. 



5. Sodium asparaginate 1-0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, and 5 in 1. 

 Sterilization: Method not given. 

 Use : Cultivation of actinomycetes. 

 Reference: Conn (1921 p. 7). 



1514. Beijerinck's Malate Asparagin 

 Agar 



Constituents : 



1. Water. 



2. Agar. 



3. Sodium malate. 



4. Asparagin. 



5. Potassium phosphate. 



6. Mohr salt (NH4)2S04 

 Preparation : 



(1) Prepare a water solution of agar that 

 has been washed free of soluble 

 material with distilled water. 



(2) Pour distilled water over the solidified 

 mass and renew the water often. 



(3) Melt and boil. 



(4) Add a small amount of nutrient solu- 

 tion consisting of sodium malate, 

 asparagin and potassium phosphate 

 (amounts not specified). 



(5) Boil again to remove all the air. 



(6) Add a drop of neutral solution of 

 Mohr salt, (NH4)2S04, containing a 

 trace of Na^COs, there should be no 

 turbidity. 



Sterilization: Not specified. 



Use: Cultivation of Spirillum desulfuri- 



cans, Spirillum tenue and other sulphate 



reducing organisms. 

 Reference: Beijerinck (1895 p. 108). 



1515. Fraenkel's Lactate Asparagin Agar 

 (Klimmer) 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 2.0 g. 



3. NaCl 5.0 g. 



4. Ammonium lactate... 6.0 g. 



5. Asparagin 4.0 g. 



6. Agar 15.0 to 30.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, and 6 in 1. 



(2) Make distinctly alkaline (indicator 

 not specified). 



Sterilization: Not specified. 

 Use: Synthetic culture medium. 

 Reference: Klimmer (1923 p. 172). 



1516. Voges' Lactate Asparaginate Agar 



Same as medium 4416, but containing 10.0 

 g. of agar per liter of medium. 



