CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



441 



1517. Giltay's Citric Acid Asparagin Agar 



(Lohnis) 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. KNO3 2.0 g. 



3. Asparagin 10 g. 



4. Citric acid 50g 



5. KH2PO4 2^0 g 



6. MgS04 2.0 g. 



7. CaClj 0.2 g. 



8- FeaCle trace 



9. Agar (1.5%) I5.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 250.0 cc. of 1. 



(2) Dissolve 4, 5, 6, 7 and 8 in 500.0 cc. 

 of 1. 



(3) Neutralize (2) by the addition of 

 KOH. 



(4) Mix (1) and (3) and make up to a 

 liter. 



(5) Dissolve 1.5% agar in (4). 



(6) Tube. 



Sterilization: Method not given. 

 Use: To study denitrification. 

 Reference: Lohnis (1913 p. 98). 



1518. Conn's Asparaginate Agar 

 Constituents: 



1. Distilled water 1000.0 cc 



2. Agar 12.0 g. 



3. Glucose 1.0 g 



4. Sodium asparaginate 1.0 g 



5. Glycerol 10.0 g. 



6. NH4H2PO4 1.5 g 



7. CaCl2 0.1 g 



8. MgS04 0.2 g 



9. KCl 0.1 g 



10. FeCls trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8, 9 and 10 in 1. 



(2) Reaction adjusted to 1.0% N acid to 

 phenolphthalein. 



Sterilization: Method not given. 



Use: Cultivation of soil microorganisms. 



Variants: Giltner used 15.0 g. agar and 



specified the use of fused CaCU and 



MgS04-7H20. 

 References: Conn (1917 p. 42), Giltner 



(1921 p. 373), Harvey (1921-22 p. 102). 



1519. Smith's Levulose Asparaginate Agar 

 Constituents: 



1. Water 1000.0 cc. 



2. Levulose 20.0 g. 



3. Glycerol lo.o g. 



4. Asparagin 1.0 g. 



5. Tannin (preferably from 

 Sumack) 1.0 g. 



6. Potassium citrate 1.0 g. 



7. Agar 2O.O g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 

 Sterilization: Method not given. 

 Use: Cultivation of B. Acaciae. Author 



reported that slime was produced. 

 Reference: Smith (1905 p. 383). 



1520. Higgins' Basic Lactose Asparagin 

 Agar 



Solidify Basal Medium 386 by the addition 

 of agar. 



1521. Sullivan's Glycerol Nitrate Asparagin 

 Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Asparagin 10.0 g. 



3. MgS04 0.2 g. 



4. K2HPO4 1.0 g. 



5. Ammonium lactate 0.5 g. 



6. NaCl 5.0 g. 



7. KNO3 0.2 g. 



8. Glycerol 10.0 g. 



9. Agar 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 

 Sterilization: Not specified. 

 Use: General Synthetic Medium. 

 Reference: Sullivan (1905-06 p. 120). 



1522. Maassen's Malic Acid Asparagin Agar 

 (Klimmer) 



1. Water 1000.0 cc. 



2. MgS04 (crystalline) 0.4 g. 



3. Asparagin 10.1 g. 



4. CaCl2 0.01 g. 



5. Na2HP04 2.0 g. 



6. NasCOg (crystal- 

 line) 2.5 g. 



7. Glucose 5.0 to 10.0 g. 



8. Malic acid 7.0 g. 



9. Agar 15.0 to 30.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 

 Sterilization: Not specified. 

 Use: Synthetic culture medium. 

 Reference: Klimmer (1923 p. 172). 



