442 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



1523. Uschinsky's Glycerol Asparaginate 



Agar (Klimmer) 

 Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 2.0 to 2.5 g. 



3. MgS04 (Crystalline) . 0.2 to 0.4 g. 



4. NaCl 5.0 to 7.0 g. 



5. Ammonium lactate... 6.0 to 7.0 g. 



6. CaCh 0.1 g. 



7. Sodium asparaginate. 3.5 g. 



8. Glycerol 30.0 to 40.0 g. 



9. Agar 15.0 to 30.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 



Sterilization: Not specified. 



Use: Synthetic culture medium. 



Reference: Klimmer (1923 p. 172). 

 1524. Berthelot's Nitrate Tyrosine Agar 

 Same as medium 402, but solidified by the 



addition of agar. 



1525. Jones' Histidin-hydro-chloride Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Histidin-hydro-chloride . . 1.0 g 



3. KNO3 0-25 g 



4. CaCl2 002g 



5. K2SO4 0.2 g 



6. MgS04 0-2 g 



7. K-2HP04 5.0 g 



8. Agar 8.0 g 



9. NajSO 3 (10.0% solution). 12.0 cc. 

 10. Basic fuchsin solution 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 500.0 cc. of 

 distilled water. 



(2) Dissolve 6 in 100.0 cc. distilled 

 water. Add to (1). 



(3) Dissolve 7 in distilled water. 



(4) Mix (2) and (3) and make up to 

 1000.0 cc. 



(5) Wash 8.0 g. agar and add to (4). 



(6) Sterilize at 15 pounds pressure for 

 15 minutes. 



(7) Add 12.0 cc. of a 10.0% NajSOs 

 solution. 



(8) Titrate to neutral to litmus using 

 N/10 HCl. 



(9) Add 30 drops of saturated alcoholic 

 solution of basic fuchsin. 



(10) Mix thoroly and pour into plates. 

 Sterilization: Sterilization given in step 

 (6) of preparation. 



Use: Isolation of B. aminophilu. Author 

 reported that B. aminophilus developed a 

 colony 2.3 mm. in diameter, after 24 hours 

 at 37°C., having the appearance of a 

 clear colorless drop of water. 



Reference: Jones (1918 p. 127). 



1526. Sohngen's Malate Urea Agar 

 (Percival) 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Calcium malate 5.0 g. 



3. K2HPO4 , 0.5 g. 



4. Urea 10.0 g. 



5. Agar 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



Sterilization: Not specified. 



Use: Cultivation of urea organisms from 

 soil and urine. Author reported that 

 urea splitting organisms were surrounded 

 with small white crystals of calcium 

 carbonate. 



Reference: Percival (1920 p. 225). 



1527. Perotti's Glucose Dicyandiamide Agar 

 Same as medium 483 but solidified by the 

 addition of 1.5% agar. 



1528. Stapp's Uric Acid Agar 



Constituents: 



1. Water lOCO.O cc. 



2. Uric acid 0.5 g. 



3. Na2HP04 3.0 g. 



4. KH2PO4 10 g. 



5. CaCl2 0.1 g. 



6. MgS04-7H20 0.3 g. 



7. NaCl 0.1 g. 



8. Fe2Cl6 0.01 g. 



9. Agar (2.0%) 20.0 g. 



Preparation : 



(1) Dissolve 4, 5, 6, 7 and 8 in 100.0 cc. of 

 water. (Meyers solution). 



(2) Dissolve 2 and 3 in 450.0 cc. of water. 



(3) Mix 50.0 cc. of (1) and (2) and solidify 

 by the addition of 2.0% agar. 



(4) Distribute in 50.0 cc. lots in 200.0 cc. 

 Erlenmeyer flasks. 



Sterilization: Method not given. 



Use: Isolation of uric acid splitting bac- 

 teria from feces and soil, Bac. cobayae, 

 Bac. capri, Bac. guano, Bac. musculi, 

 Bac. hollandicus. 



Reference: Stapp (1920 p. 3). 



