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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Boekhout and de Vries Tartrate 

 Peptone Agar 1576 



Dawson's Butter Soap Peptone 

 Agar 1577 



Harvey's Phenol Peptone Agar 1578 



Da. More than one type of additional 



organic carbon supplied. 

 El. Carbohydrates and alcohols added. 



Bacto Malt Extract Agar (Synthe- 

 tic) (Dehydrated) 1579 



Harvey's Glucose Glycerol Agar — 1580 

 Ej. Carbohydrates and organic acids added. 



Beijerinck's Glucose Peptone Agar. 1581 



Sarbouraud's Glucose Peptone Agar 

 (Serena) 1582 



Chantemesse's Phenol Peptone Agar 

 (Bezan5on) 1583 



Boekhout and de Vries' Maltose 

 Peptone Agar 1584 



Cheyney's Maltose Peptone Agar... 1585 



Sabouraud's Glycerol Peptone Agar 1586 

 Ea. Other combinations of organic carbon 



added. 



Robinson and Rettger's Glycerol 

 Opsine Citrate Agar 1587 



Botelho's Lacto-phenol Peptone 



Agar 1588 



Cj. Containing nitrogen in addition to 



peptone. 

 Di. Inorganic nitrogen added. 



Sullivan's Ammonium Lactate Pep- 

 tone Agar 1589 



Lipman and Brown's Nitrate Pep- 

 tone Agar 1590 



Beijerinck's Ferric Ammonium Ci- 

 trate Peptone Agar (Janke) 1591 



Vierling's Nitrate Peptone Agar 1592 



Heinemann's Asparagin Peptone 



Agar. 1593 



Dj. Inorganic nitrogen not added; addi- 

 tional organic nitrogen supplied. 



MacConkey's Basal Bile Salt Pep- 

 tone Agar 1594 



Harrison and van der Leek's Aescul- 

 in Bile Salt Agar 1595 



Harvey's Brilliant Green Bile Salt 

 Agar 1596 



1530. Jacobi's Peptone Agar 



Constituents : 



1. Water 1500.0 cc. 



2. Meat peptone (Kem- 

 merich's) 7.5 g. 



3. Peptone (siccum) 15.0 g. 



4. Agar-agar 15.0 to 22.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 by heating 

 over a free flame. 



(2) Make up to the original volume by 

 the addition of water. 



(3) Add Na2C03 or sodium phosphate 

 until the reaction is slightly alkaline. 



(4) Pour into a flask and steam until the 

 albuminous material still to be 

 removed is completely separated. 

 (Usually 2 hours if sodium phosphate 

 is used and longer if NajCOs.) 



(5) Filter thru cotton, using compressed 

 air to effect a fast filtration. 



(6) Distribute into smaller flasks. 



(7) Distribute sterile (6) into sterile test 

 tubes that have been autoclaved in 

 the autoclave for 2J hours. (The 

 tubes are contained in an enamel 

 container in the autoclave. The 

 inner temperature reaching about 

 150°C.) 



Sterilization: Heat (6) in streaming vapor 

 for two hours (if the glassware used has 

 been sterilized in the autoclave). 



Use: General culture medium. 



Reference: Jacobi (1888 p. 538). 



1531. Molisch's Manganese Peptone Agar 



Constituents: 



1. Water (Moldau River) 1000.0 cc. 



2. Manganese peptone 0.5 g. 



3. Agar 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Pour in plates. 

 Sterilization: Method not given. 



Use: Cultivation of iron bacteria, Lepto- 



thrix ochracea. 

 Reference: Molisch (1910 p. 36). 



1532. Roux and Rochaix' Peptone Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Agar 25.0 g. 



3. Peptone 10.0 to 15.0 g. 



Preparation : 



(1) Soak 25.0 g. of chopped agar for 24 

 hours in 500.0 cc. of water acidulated 

 by the addition of 6.0% HCl. Stir 

 occasionally. 



(2) Wash thoroly with water. 



