CULTURE MEDIA FOR CULTIVATION OF MICRO ORGAXISMS 



445 



(3) Soak the agar for 24 hours in 500.0 

 cc. of a 5.0% ammonia solution. 



(4) Wash thoroly. 



(5) Place in a liter of distilled water and 

 heat until the agar is dissolved. 



(6) Add 50.0 cc. of water containing 10.0 

 to 15.0 g. peptone. 



(7) Neutralize by the addition of a 

 saturated solution of NaHCOa. 



(8) Pass thru flannel and then filter using 

 a hot water funnel. 



(9) Distribute into flasks or tubes. 

 Sterilization: Sterilize at 115° to 120° for 



30 minutes. 

 Use: General culture medium. 

 Variants : 



(a) Noyes dissolved 15.0 g. of the best 

 agar and 0.05 g. of Witte's peptone in 

 1000.0 cc. of water. 



(b) Harvey dissolved 18.0 g. of agar and 

 30.0 g. peptone in 1000.0 cc. of water. 

 This medium was used to cultivate 

 hyphomycetes. 



(c) Mortensen solidified medium 560 by 

 the addition of 2.0 or 2.5% agar. 



(d) Moll used a 2.0% Witte peptone 

 solution solidified with 2.0% agar as 

 a basal medium and added a variety 

 of inorganic salts to study the effect 

 of salts on the growth of molds. He 

 reported that the toxicity of salts is 

 an additive characteristic of the 

 ion. The toxicity depends on the 

 solubility and the ionization of the 

 salt. 



References: Roux and Rochaix (1911 p. 

 115), Noyes (1916 pp. 93-94), Harvey 

 (1921-22 p. 102), Mortensen (1909 p. 523), 

 Moll (1920 p. 258). 



1533. Hesse and Niedner's Nahrstoff- 

 Heyden Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Agar 12.5 g. 



3. NahrstofT-Heyden 7.5 g. 



Preparation : 



(1) Mix 3 with a little cold distilled water 

 forming a paste. 



(2) Dissolve 2 and (1) in 1. 



(3) Filter thru cotton or paper in the 

 steamer or hot water funnel. 



(4) Adjustment of reaction not required. 



(5) Pour in plates. 



Sterilization: Not specified. 



Use: The authors used the medium pri- 

 marily for water analysis, but various 

 investigators have used the same or 

 similar media for a large variety of 

 purposes. 



Variants : 



(a) Wimmer used the medium with only 

 980.0 cc. water instead of 1000.0 cc. 

 to study nitrification. 



(b) Klimmer used 8.0 g. Nahrstoff- 

 Heyden and 13.0 g. agar. 



References: Hesse and Niedner (1898 pp. 

 454-462), Wimmer (1904 p. 139), Smith 

 (1905 p. 196), Tanner (1919 p. 50), Percival 

 (1920 p. 121), Klimmer (1923 p. 194). 



1534. Lichtenstein's Yeast Extract Agar 



Same as medium 518, but solidified by the 

 addition of agar. 



1535. Banning's Basal Salt Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. KH.PO4 3.0 g. 



3. MgS04 2.0 g. 



4. Peptone 10.0 g. 



5. Agar 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Do not adjust the reaction. 



(3) Dissolve one of the added nutrients 

 in (1). 



Sterilization: Heat to 75°C. to sterilize 



(method not given). 

 Use: To study o.xalic acid formation by 



Bad. xylinum, Bad. aceti oxalici and 



Bad. diabeticum. Author reported that 



when nitrogenous materials were added 



oxalic acid was not produced. 

 Added nutrients: The author added one 



of the following materials: 



methyl alcohol, 10.0 g. 



ethyl alcohol " 30.0 g. 



propyl alcohol 20.0 g. 



butyl alcohol 10.0 g. 



amyl alcohol 5.0 g. 



ethylene glycocol 10.0 g. 



glycerol 10.0 g. 



erythritol 10.0 g. 



mannitol 10.0 g. 



sodium salt of acetic acid 10.0 g. 



propionic acid 10.0 g. 



butyric acid 5.0 g. 



