446 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



isobutyric acid 5.0 g. 



valearic acid 5.0 g. 



glyoxalic acid 2.5 g. 



lactic acid 10.0 g. 



malonic acid 2.5 g. 



benzoic acid (potassium salt) 5.0 g. 



salicilic acid 5.0 g. 



pyrotaric acid 2.5 g. 



malic acid 10.0 g. 



tartaric acid. 10.0 g. 



salicylic acid 5.0 g. 



glycocol (sodium salt) 10.0 g. 



leucine (sodium salt) 10.0 g. 



hippuric acid 5.0 g. 



sarcosine 2.5 g. 



uric acid (potassium salt) 5.0 g. 



Urea 10.0 g. 



creatin 2.5 g. 



creatinin 2.5 g. 



glucose 20.0 g. 



levulose 20.0 g. 



galactose 20.0 g. 



maltose 20.0 g. 



sucrose 20.0 g. 



lactose. 20.0 g. 



raffinose^. 20.0 g. 



rhamnose 10.0 g. 



arabinose 20.0 g. 



starch (wheat) 10.0 g. 



inulin 10.0 g. 



glycogen 10.0 g. 



dextrin 10.0 g. 



gum arable 10.0 g. 



Reference: Banning (1902 p. 395-427). 



1536. Matzuschita's Basal Sodium Chloride 

 Peptone Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar (2.0%) 20.0 g. 



3. Peptone (1.0%) 10.0 g. 



4. NaCl (0.5%) 5.0 g. 



Preparation: 



(1) Prepare a watery infusion of one of 

 the materials given under added 

 nutrients. 



(2) Add 2.0% agar, 1.0% peptone and 

 0.5%o NaCl to (1). 



(3) Neutralize (indicator not given). 

 Sterilization: Not specified. 



Use: Cultivation of intestinal bacteria. 

 Similar media were used for the cultiva- 

 tion of various other organisms. 



Added nutrients: The author prepared 



infusion from one of the following 

 materials: 



intestinal mucosa feces (human) 



liver brain 



pancreas rice 



spleen pea 



Variants : 



(a) The author used undiluted bile, 

 urine or beer wort instead of infusion 

 listed above. 



(b) Matzuschita cultivated spore forming 

 bacilli, Clostridium butyricum, 

 Bacillus oedematis maligni, Bacillus 

 anthracis syniptomatici, Bacillus 

 sporogenes, Bacillus botulinus, on a 

 medium prepared as follows: 



(1) Dissolve 10.0 g. Koch's meat pep- 

 tone, 20.0 g. agar, 5.0 g. NaCl, 

 and 2.0% glucose (the glucose may 

 be omitted) in 1000.0 cc. water by 

 boiling in a steamer. 



(2) Neutralize. 



(3) Filter. 



(4) Sterilize in the steamer on from 2 to 

 5 successive days for 15 to 30 

 minutes. 



(5) Incubate for 2 days at 37°C. to test 

 sterility. 



(c) Sullivan used the basal solution with 

 20.0 g. peptone instead of 10.0 g., 

 as a general culture medium for 

 Microspira comma, B. anthracis, B. 

 pneumoniae, photogenic and chromo- 

 genic bacteria. He reported slow 

 growth and slight pigment pro- 

 duction. 



(d) Harvey used the basic solution with 

 10.0 to 20.0 g. peptone added. He 

 merely stated that the solution be 

 solidified by the addition of agar, not 

 giving the amount employed. 



(e) Teague and Deibert reported that a 

 medium containing peptone, NaCI 

 and agar would not support the 

 growth of Unna-Ducrey bacillus. 



References: Matzuschita (1901-02 p. 214), 

 (1902 p. 288), Sullivan (1905-06 p. 114), 

 Harvey (1921-22 p. 101), Teague and 

 Deibert (1922 p. 70). 



1537. Glaessner's Nahrstoff-Heyden Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar (pulverized) 15.0 g. 



