CULTURE MEDIA FOR CULTIVATION" OF MICROORGANISMS 



447 



3. Nahrstoff-Heyden 10.0 g. 



4. XaCl,.., 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Method not given. 

 Use: Cultivation of diphtheria bacilli. 



Author reported better growth on 



Loeffler's blood serum. 

 Reference: Glaessner (1900 p. 729). 



1538. Crendiropoulo and Panayotatou's 

 Alkaline Peptone Agar 



Constituents : 



1. Water 190.0 cc. 



2. Peptone 5.0 g. 



3. NaOH (10.0%) 10.0 cc. 



[agar 3.0 g. 



4. Agar j peptone 1-0 g. 



medium ] NaCl 0.5 g. 



[HjO 100.0 cc. 



Preparation : 



(1) Dissolve 5.0 g. of peptone (Witte or 

 Chepoteau) in 190.0 cc. of water. 



(2) Add 10.0 cc. of 10.0% solution of 

 NaOH (8.0 cc. if using Witte's pep- 

 tone) and heat 3 to 5 minutes. 



(3) Cool and filter thru paper. 



(4) Prepare neutral peptone agar by 

 dissolving 3.0 g. agar, 1.0 g. peptone 

 and 0.5 g. NaCl in 100.0 cc. water. 



(5) Mi.x 4 parts sterile (4) with 6 parts 

 sterile (5) and pour in sterile plates. 



Sterilization: Sterilize (4) at 100°C. for 30 

 minutes. Method of sterilization of (5) 

 not given. 



Use: Diagnosis of cholera. Author re- 

 ported that vibrio colonies are round, 

 semi-transparent, bluish at first and later 

 whitish. 



Reference: Crendiropoula and Panayota- 

 tou(1910p. 249). 



1539. Stutzer and Hartleb's Phosphate 

 Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 20.0 g. 



3. Peptone 20.0 g. 



4. Potassium phosphate 1-0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Neutralize by the addition of soda 

 (indicator not specified). 



(3) Add0.5g. NasCOsto (2). 



Sterilization: Method not given. 



Use: Used by Stutzer and Hartleb in an 



attempt to cultivate the foot and mouth 



disease bacterium. Temple and others 



used similar media to determine bacterial 



counts in soil. 

 Variants: Temple used 0.1 g. K2HPO4, 1.0 



g. peptone and 15.0 g. of agar per liter. 

 References: Stutzer and Hartleb (1897 p. 



404), Temple (1912 p. 206), Brown (1913 



p. 499). 



1540. Molisch's Salt Peptone Agar 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. MgS04 0.5 g. 



3. K2HPO4 0.5 g. 



4. FeS04 trace 



5. Peptone 10.0 g. 



6. Agar 18.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of purple bacteria. 

 Reference: Molisch (1907 p. 11). 



1541. Bacto Sabouraud's Dextrose Agar 

 (Dehydrated) 



Constituents: 



1. Water 



2. Peptone (Bacto) 10.0 g. 



3. Glucose (Bacto) 40.0 g. 



4. Agar (Bacto) 15.0 g. 



Preparation : 



(1) Dissolve 65.0 g. of Bacto Sabouraud's 

 Dextrose Agar (dehydrated) in 1000.0 

 cc. of distilled water by boiling. 



(2) Distribute as desired. 



(3) If sterilized for 20 minutes at 15 

 pounds pressure pH = 5.6±. 



Sterilization: Sterilize as desired. Avoid 

 e.xcess heat on account of high acidity of 

 medium. 



Use: General culture medium. 



Reference: Digestive Ferments Co. (1925 

 p. 14). 



1542. Sabouraud's Glucose Peptone Agar 

 (Anderson) 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 20.0 g. 



3. Peptone 10.0 g. 



4. Glucose 40.0 g. 



