448 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Adjust to +2 acid with N/1 HCl. 



(3) Add 4. 



(4) Tube in sterile tubes. 

 Sterilization: Sterilize for 10 minutes at 5 



pounds pressure. 

 Use: To isolate yeast found in human 



intestinal tract. Different investigators 



used similar media to cultivate yeast, 



molds and other organisms. 

 Variants : 



(a) BezanQon used 18.0 g. agar instead of 

 20.0 g. The medium was used to 

 cultivate hyphomycetes, Sporotri- 

 chum beurmanni . 



(b) Harvey cultivated tineae, molds and 

 eporothrix on a medium prepared as 

 follows: 



(1) Prepare: Granulated peptone 10; 

 commercial glucose 40; agar 18; 

 water 1000. 



(2) Raise slowly in the autoclave to a 

 temperature of 120°C. and then 

 allow to fall to 100°C. 



(3) Shake, to mix, at a temperature of 

 100 °C. 



(4) Have in readiness 2 one-liter flasks 

 with funnels and thick, filter 

 paper. 



(5) Place these flasks in the hot 

 autoclave. 



(6) Filter. 



(7) Keep the unfiltered medium hot. 



(8) Replace the funnels and filter 

 paper by new funnels with filter 

 paper as soon as filtration becomes 

 slow. 



Note: The filtration of this 

 medium is particularly difficult 

 and slow. 



(9) Distribute the filtrate into test 

 tubes. 



Note: The tubes should be 

 capped during incubation (which 

 may be long) but may be placed 

 in a covered receptacle with its 

 cover only just open. 

 (10) No adjustment of reaction of this 

 medium required. 

 References : Anderson (1917 p. 343), Tanner 

 (1919 p. 51), Bezangon (1920 p. 646), 

 Harvey (1921-22 pp. 101, 110). 



1543. Matzuschita's Glucose Peptone .4.gar 



Constituents : 



1. Water lOOO.O cc. 



2. Peptone. 10.0 g. 



3. NaCL......" 5.0 g. 



4. Agar 12.0 g. 



5. Glucose (2.0%) . , 20.0 g. 



Preparation : 



(1) Prepare ordinary nutrient agar using 

 water instead of bouillon. (Peptone 

 and NaCl in amounts indicated were 

 assumed to be the constituents of 

 nutrient agar.) 



(2) Add 2.0% glucose to (1). 

 Sterilization: Not specified. 



Use: Cultivation of mammalian and 

 chicken tubercle bacilli. Author re- 

 ported that the mammalian types grew 

 poorly. Chicken types gave colonies of 

 1.5 mm. after six days. 



Variants : 



(a) Truche and Cotoni solidified variant 

 (a) medium 596 by the addition of 

 agar. 



(b) Harvey solidified medium 595 with 

 agar. 



References: Matzuschita (1899 p. 128), 

 Truche and Cotoni (1911 p. 480), Harvey 

 (1921-22 p. 109). 



1544. Glaessner's Glucose Peptone Agar 



Same as medium 1537 but contains 1.0% 

 glucose. The author also described a 

 medium the same as 1537, containing in 

 addition 10.0 g. peptone and 10.0 g. glucose. 



1545. Hucker and Wall's Glucose Peptone 

 Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 40.0 g. 



3. Glucose 2.0 g. 



4. K2HPO4 5.0 g. 



5. Agar 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Tube. 



Sterilization: Method not given. 



Use : To determine ammonia production by 

 organisms. Add 1.0 cc. of a 10.0% 

 phenol and 1.0 cc. of a 1.0% (available 

 chlorine) sodium hypochlorite solution 



