CULTURE MEDIA FOR CULTIVATIOX OF MICROORGANISMS 



449 



to the surface of the agar slant. A blue 

 color developing within 30 minutes 

 denotes ammonia production. 

 Reference: Hucker and Wall (1922 p. 516), 

 (1922 p. 485). 



1546. Hesse and Niedner's Glucose Peptone 

 Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar 12.5 g. 



3. Peptone (Gehe & Co.) 10.0 g. 



4. De.xtrose 10 g. 



5. Sodium phosphate (ampho- 

 teric) 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 0.5 g. amphoteric 

 reacting sodium phosphate in 1 by 

 boiling. 

 Sterilization: Not specified. 

 Use: Used in water analysis. 

 Reference: Hesse and Niedner (1898 pp. 

 454-462). 



1547. Levine's Boric Acid Peptone Agar 



Constituents: 



1. Water 1000.0 g. 



2. Peptone (1.0%) 10.0 g. 



3. Agar (1.5%) 15.0 g. 



4. K2HPO4 (0.3%) 3.0 g. 



5. Glucose (0.05%) 0.5 g. 



6. Boric acid (0.63%) 6.3 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 

 Sterilization: Not specified. 

 Use: Differentiation of Bad. coli and Bad. 



aerogenes. Author reported that Bad. 



aerogenes did not show growth. Bad. 



coli grew luxuriantly. 

 Reference: Levine (1911 p. 22). 



1548. Bacto Lead Acetate Agar 

 (Dehydrated) 



Constituents : 



1. Distilled water 



2. Peptone (Bacto) 10.0 g 



3. Agar (Bacto) 12.0 g 



4. Glucose (Bacto) 1.0 g 



5. NaCl...., 5.0 g 



6. Lead acetate 0.1 g 



Preparation : 



(1) Dissolve 20.0 g. of Bacto Lead Acetate 

 Agar (dehydrated) in 1000.0 cc. of 

 distilled water. 



Sterilization: Sterilize in the usual manner. 

 Use: Primarily used for differentiating 



Sal. paratyphi from Sal. schotmuelleri . 



Used also in the study of other organisms 



of this group. 

 Reference: Digestive Ferments Co. (1925 



p. 12). 



1549. Lipman and Brown's Glucose Peptone 

 Agar 



Constituents : 



1. Water (tap) 1000.0 cc. 



2. Dextrose 10.0 g. 



3. K2HPO4 0.5, 1.0 or 1.5 g. 



4. MgS04 0.2 g. 



5. Peptone 0.05 g. 



6. Agar 20.0 g. 



Preparation : 



(1) Dissolve 2, 4, 5 and 6 in 1. 



(2) To 1000.0 cc. of (1) add 0.5 g. of 

 K2HPO4. This is medium A. 



(3) To lOOO.O cc. of (1) add 1.0 g. K2HPO4. 

 This is medium B. 



(4) To 1000.0 cc. of (1) add 1.5 g. K2HPO4. 

 This is medium C. 



(5) To 1000.0 cc. of (1) add 0.5 g. K2HPO4, 

 and 0.5 cc. of N/1 HCl. This is 

 medium D. 



(6) To 1000.0 cc. of (1) add 0.5 g. K2HPO4 

 and 1.0 cc. N/1 HCl. This is me- 

 dium E. 



Sterilization: Not specified. 



Use: Bacterial count of soil. Author re- 

 ported that Media A, B, D and E or 

 combinations (2), (3), (5) and (6) gave 

 practically the same results. The re- 

 action with 1.5 g. K2HPO4 seemed to 

 inhibit development. 



Variants : 



(a) Authors used 0.5 g. K2HPO4 and 0.1 

 to 0.5 g. peptone, instead of amounts 

 used above. 



(b) Waksman used 1.0 g. KH2PO4, 5.0 g. 

 peptone and 25.0 g. agar instead of 

 amounts used by Lipman and Brown. 



References: Lipman and Brown (1910 pp. 

 447, 451, 592), Tanner (1919 pp. 49, 66), 

 Heinemann (1922 pp. 38, 40), Waksman 

 (1922 p. 340). 



1550. Waterman's Glucose Peptone Agar 



Constituents : 



1. Agar solution 1000.0 cc. 



2. Glucose (2.0%) 20.0 g. 



