450 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Peptone (0.2%) 2.0 g. 



4. KH0PO4 (0.1%) 1.0 g. 



5. CaCOa (0.2%) 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1000.0 cc.of 

 agar solution. 



Sterilization: Method not given. 



Use: To study acid production by acetic 

 acid bacteria, Acetobacter melanogenum, 

 B. Pasteurianum, B. rancens, B. xylinum 

 and acetic acid bacteria from beer. B. 

 xylinum and Acetobacter melanogedum 

 produced acid. The remaining bacteria 

 listed produced no or very little acid. 



Variants : Janke gives this medium specify- 

 ing that the agar be a 2.0% agar solu- 

 tion in water. 



References: Waterman (1913 p. 453), Janke 

 (1916 p. 6). 



1551. Pfeiler and Lentz's Ringer Solution 

 Agar 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 10.0 g. 



3. KCl 0.2 g. 



4. CaCl2 0.2 g. 



5. Sodium bicarbonate 0.1 g. 



6. Glucose 1.0 g. 



7. Agar 15.0 g. 



8. Peptone 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add agar and peptone to (1) and boil 

 three hours. 



(3) Cool to 50°C. and clarify with egg 

 albumin powder. Add albumin, boil 

 and filter. 



Sterilization: Method not given. 



Use: General inexpensive culture medium. 

 Author reported that organisms retained 

 their characteristic growth, their patho- 

 genicity, their ability to produce pigment, 

 their agglutinative ability and their 

 gram stain on this medium. (Messer- 

 schmidt in Centr. f. Bakt., 68: 107-111 

 1913, compares this medium with meat 

 extract agar and finds meat extract 

 medium far superior. This medium does 

 not give characteristic reaction when 

 used as a base for Endo Fuchsin agar, 

 Conradi-Drigalski agar or Loeffler's Mala- 

 chite green agar according to Messer- 

 schmidt.) 



Variants: Park, Williams and Krumwiede 

 used 1.0 g. NaHCOa with 1.0 or 2.0% 

 peptone and 1.5 or 2.0% agar instead of 

 amounts indicated above. 



References: Pfeiler and Lentz (1913 p. 123), 

 Park, Williams and Krumwiede (1924 p. 

 122). 



1552. Harden's Glucose Peptone Agar 



Constituents : 



1. Water 500.0 cc. 



2. Glucose 10.0 g. 



3. Peptone (Witte) 5.0 g. 



4. Chalk 5.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add sterile 4 to sterile (1). 

 Sterilization: Sterilize (1); method not 



given. 

 Use: To study fermentation of glucose. 

 Reference: Harden (1905 p. 488). 



1553. Greig-Smith's Sucrose Peptone Agar 



Same as medium 613 but solidified by the 

 addition of 2.0% agar. 



1554. Owen's Raw Sugar Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar (2.0%) 20.0 g. 



3. Sucrose (second 80°) 

 (10.0%) 100.0 g. 



4. Peptone (1.0%) 10.0 g. 



Preparation: 



(1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 

 Use: Bacterial count of cane sugar 



products. 

 Reference: Owen (1914 p. 338). 



1555. Vierling's Sucrose Peptone A^ar 



Same as 622 but solidified by the addition 

 of 2.0% agar. 



1556. Levine's Eosine Methylene Blue Agar 

 (Dehydrated) 



Constituents: 



1. Distilled water 



2. Peptone, Bacto 10.0 g 



3. Agar, Bacto 15.0 g 



4. K2HPO4 2.0 g 



5. Lactose, Bacto 10.0 g 



6. Eosine 0.4 g 



7. Methylene Blue 0.1 g 



