452 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



basic fuchsin, and 2.5 cc. of a freshly 

 prepared 10.0% Na2S03 solution to 

 each 100.0 cc. of sterile (4). 

 (6) Pour into plates and allow to harden 

 in the incubator. 

 Use: Direct isolation or confirmation of 

 presumptive test for lactose fermenters. 

 Variants : 



(a) Harvey specified the use of 3.5 g. 

 K0HPO4 and 20.0 g. agar. 



(b) Harris specified the use of 3.5 g. 

 K2HP04and 15.0 g. agar. 



References: Levine (1921 p. 115), Harvey 

 (1921-22 p. 93), Harris (1925 p. 280). 



1560. Robin's Lactose Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 32.0 g. 



3. Peptone (Collas) 20.0 g. 



4. Sodium phosphate 0.4 g. 



5. Blue, soluble 



6. Lactose 40.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 by heating at 

 115° in the autoclave for 5 to 10 

 minutes. 



(2) Add 1.0 cc. of a 1.0% aqueous solution 

 of soluble blue to boiling (1). 



(3) Add sufficient tenth normal KOH 

 solution to decolorize (2). 



(4) Add 40.0 g. lactose to (3). 



(5) Filter. 



(6) Distribute in tubes. 

 Sterilization: Sterilize at 105°C. for 15 



minutes. 



Use: Differentiation of coli and typhoid 

 bacilli. Author reported that coli colo- 

 nies colored the medium blue while ty- 

 phoid colonies were colorless. 



Reference: Robin (1897 p. 50). 



1561. Harvey's Lactose Peptone Agar 



Same as medium 607 but solidified by the 

 addition of agar. 



1562. Sabouraud's Maltose Peptone Agar 



(Wurtz) 



Constituents : 



1. Water..,., 1000.0 cc. 



2. Maltose ^, 38.0 g. 



3. Peptone 5.0 to 8.0 g. 



4. Agar 14.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 as in the 

 preparation of ordinary nutrient 

 agar. 

 Sterilization: Method not given. 

 Use: Cultivation of molds. 

 Variants : 



(a) Harvey used 18.0 g. agar, 40.0 g. of 

 commercial French maltose and 10.0 

 g. of Chassaing peptone. 

 References: Wurtz (1897 p. 47), Harvey 

 (1921-22 p. 110), Stitt (1923 p. 208). 



1563. Molisch's Dextrin Peptone Agar 



Constituents : 



1. Water (Moldau) 1000.0 cc. 



2. Agar... 18.0 g. 



3. Peptone 5.0 g. 



4. De.xtrin. ., 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Method not given. 

 Use: Cultivation of purple bacteria. 

 Reference: Molisch (1907 p. 11). 



1564. Noyes' Starch Peptone Agar 



Constituents: 



1. Water.,, 1000.0 cc. 



2. Agar (best) 15.0 g. 



3. Starch 2.0 g. 



4. Peptone...., 0.05 g. 



Preparation: 



(1) Dissolve 2, 3 and 4 in 1. 



Sterilization: Not specified. 



Use: General culture medium. Used pri- 

 marily to study soil organisms. 



Reference : Noyes (1916 pp. 93, 94). 



1565. Vierling's Starch Peptone Agar 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. CaClo 0.1 g. 



4. MgS04 0.1 g. 



5. FeCls,. trace 



6. NaCl..,.., trace 



7. Starch 5.0 g. 



8. Peptone 10.0 g. 



9. Agar 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Solidify with agar (amount not 

 specified). 



Sterilization: Not given. 



