CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



453 



Use: To study amylase production by 

 mycobacteria. Author reported that the 

 agar was slightly turbid. The medium 

 was slightly cleared around mycobacteria 

 colonies. The clearing was more notice- 

 able if the starch content was increased 

 1.5 or 2.0%. The starch was dissolved. 

 No color was produced near the colonies 

 when Lugol's solution was added. 



Reference: Vierling (1920 p. 204). 



1566. Gibson's Starch Peptone Agar 



(Harvey) 



Constituents : 



1. Water. 1000.0 cc. 



2. Peptone 20.0 g. 



3. NaHCOs 1.5 g. 



4. Agar 30.0 g. 



5. Potato starch 10.0 g. 



6. Litmus 

 Preparation : 



(1) Di.s.solve 2, 3 and 4 in 1. 



(2) Filter, while hot, thru well-wetted 

 thick filter paper. 



(3) Make a suspension of 10.0 g. potato 

 starch with a little of the hot filtrate 

 and add to the bulk of the filtrate. 



(4) Mix. 



(5) Add sterile litmus solution to sterile 

 (4) to give the desired color. 



Sterilization: Sterilize (4) at 100°C. on 



each of three successive days. 

 Use: Detection of V. cholerae. 

 Reference: Harvey (1921-22 p. 112). 



1567. Bacto Eosine Methylene Blue Agar 

 (Dehydrated) 



Constituents: 



1. Distilled water 



2. Peptone, Bacto 10.0 g 



3. Agar, Bacto 15.0 g 



4. Lactose, Bacto 5.0 g 



5. Sucrose, Bacto 5.0 g 



6. K2HPO4 2.0 g 



7. Eosine 0.2 g 



8. Methylene Blue 0.05 g 



Preparation : 



(1) Dissolve 37.0 g. of Bacto Eosine 



Methylene Blue Agar (dehydrated) 



in 1000.0 cc. water by boiling or 



autoclaving. 



Sterilization: Sterilize in the usual manner. 



Use: Isolation of Esch. coli, Esch. Ebert, 



Esch. typhi, etc. 



Reference: Digestive Ferments Co. (1925 

 p. 11). 



1568. Levine's Rosolic Acid China Blue 

 Peptone Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar 15.0 g. 



3. Peptone 10.0 g. 



4. K2HPO4 4.0 g. 



5. Lactose, 20% soln 50.0 cc. 



6. Glucose, 5% soln 10.0 cc. 



7. Rosolic acid (1.0% in 90.0% 

 alcohol) 10.0 cc. 



8. China-blue (0.5% in water).. 10.0 cc. 

 Preparation : 



(1) Dis.solve 2, 3 and 4 in 1. 



(2) pH = 7.4 to 7.5, requires no adjust- 

 ment. 



(3) Need not be filtered if used on plates. 



(4) Distribute in 100.0 cc. lots. 



(5) To melted sterile (4) add 5.0 cc. 

 sterile 5, 1.0 cc. of sterile 6, 1.0 cc. of 

 7 and 1.0 cc. of 8 per 100.0 cc. 



(6) Mix thoroly and pour in sterile 

 plates. 



Sterilization: Sterilize (4), method not 

 given. 



Use: To isolate dysentery bacilli. The 

 author reported that the dyes such as 

 eosin, methylene blue, the fuchsin- 

 sulphite indicator, and an excess of 

 Rosolic acid and china blue inhibited 

 many dysentery types. 



Variants: Harvey used 5.0 g. peptone 

 instead of 10.0 g. 



Reference: Levine (1920 p. 39), Harvey 

 (1921-22 p. 88). 



1569. Sabouraud's Basal Glycerol Peptone 

 A^ar (Park, Williams & Krumwiede) 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone (1.0 to 



2.0%) 10.0 to 20.0 g. 



3. Glycerol (0.5%) 5.0 g. 



4. Agar to solidify 

 Preparation : 



(1) Dissolve 2, 3 and one of the added 

 nutrients in 1. 



(2) Solidify by the addition of agar. 



(3) Do not adjust the reaction. 

 Sterilization: Not specified. 

 Use : Cultivation of molds. 



