454 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Added nutrients: The author added 2.0% 



glucose or 2.0% maltose. 

 Reference: Park, Williams and Krumwiede 



(1924 p. 134). 



1570. Omelianski's Alcohol Peptone Agar 



Constituents : 



1. Tap water 1000.0 cc. 



2. Peptone 5.0 to 10.0 g. 



3. Potassium phosphate 1.0 g. 



4. Agar-agar 20.0 g. 



5. Ethyl alcohol few drops 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. (Details 

 of preparation not given). 



Sterilization: Method not given. 



Use: Study aroma producing microorgan- 

 isms, Bad. ester oar omaticum. Author 

 reported that more aroma was produced 

 if little alcohol be added. 



Reference: Omelianski (1923 p. 408). 



1571. Robinson and Rettger's Glycerol 

 Opsine Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Opsine 10.0 g. 



3. NaCl 5.0 g. 



4. Glycerol 50.0 g. 



Preparation : 



(1) Dissolve 2, 3, and 4 in 1 by heating. 



(2) Adjust to faintly acid or basic to 

 litmus. 



(3) Boil over flame a few minutes. 



(4) Filter and tube. 



Sterilization: Autoclave at 12 pounds pres- 

 sure for 15 minutes. 



Use: General culture medium. Author re- 

 ported that the medium would support 

 the growth of some of the pathogenic 

 forms. 



Reference: Robinson and Rettger (1918 

 p. 212). 



1572. Hesse's Glycerol Kahrstoff Heyden 

 Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Nahrstoff -Heyden 5.0 g. 



3. Glycerol 30.0 g. 



4. Agar 10.0 g. 



5. NaCl 5.0 g. 



Preparation : 



(1) Mix the Nahrstoff Heyden with a 

 little water in a beaker, forming a 

 paste. Stir until all the Nahrstoff- 

 Heyden has dissolved. 



(2) Dissolve 3, 4, 5 and 5.0 cc. of a normal 

 crystal soda solution in 1 by boiling 

 2 hours. 



(3) Mix (1) and (2). 



(4) Boil for 15 minutes, stirring con- 

 stantly. 



(5) Filter thru folded moistened filter 

 paper in a steamer. Five funnels 

 may be used to hasten the process. 



Sterilization: Not specified. 



Use: Cultivation of tubercle bacilli. 



References: Hess (1899 p. 505), Kolle and 



Wasserman (1912 p. 412), Abel (1912 p. 



94), Klimmer (1923 p. 224). 



1573. Molisch's Glycerol Peptone Agar 



Constituents : 



1. Water, Moldau 1000.0 cc. 



2. Agar 18.0 g. 



3. Peptone 5.0 g. 



4. Gh'cerol 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 

 Use : Cultivation of purple bacteria. 

 Variants: Harvey prepared Sabouraud's 



Glycerol Agar by dissolving 20.0 g. 



Chassaing's peptone, 40.0 g. glycerol and 



20.0 g. agar in 1000 cc. water. 

 Reference: Molisch (1907 p. 11), Harvey 



(1921-22 p. 101). 



1574. Spengler's Glycerol Somatose Agar 



Constituents : 



1. Distilled water 



2. Nahrstoff Heyden. 



3. Somatose 



4. NaCl 



5. Glycerol., 



6. Agar 



1000.0 cc 

 5.0 g 

 5.0 g 

 5.0 g 

 30.0 g 

 15.0 g 



7. Soda 2.0 to 4.0 g 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of tubercle bacilli. 

 References: Spengler (1903 p. 92), Kolle 

 and Wasserman (1912 p. 413). 



