CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



455 



1575. Omelianski's Formate Peptone Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Calcium formate 20.0 g. 



3. Peptone 5.0 g. 



4. Agar 15.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 

 Use: To study decomposition of formic 



acid by Bacterium formicicum. 

 Variants: Author used potassium foymate 



instead of calcium formate. 

 Reference: Omelianski (1903-04 pp. 185, 



186). 



1576. Boekhout and de Vries Tartrate 

 Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 10.0 g. 



3. Sodium tartrate 5.0 g. 



4. Agar 15.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



Sterilization: Not specified. 



Use: Cultivation of Bacillus fuchsinus. 

 Author reported that after 18 hours 

 growth was white; after 27 hours red; and 

 a metallic sheen developed after two days. 



Reference: Boekhout and de Vries (1898 p. 

 498). 



1577. Dawson's Butter Soap Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 2.5 g. 



3. Butter soap 10.0 g. 



4. Agar 20.0 g. 



Preparation: 



(1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 

 Use: Culture medium, to study variation 



of B. coli. 

 Reference: Dawson (1919 p. 142). 



1578. Harvey's Phenol Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 30.0 g. 



3. Agar 20.0 g. 



4. Carbolic acid 5.0% 0.05 g. 



Preparation: 



(1) Dissolve 2 and 3 in 1. 



(2) Add 0.05 cc. of a 5.0% phenol solution 

 to sterile melted (1) just before use. 



Sterilization: Method not given. 

 Use: General culture medium. 

 Reference: Harvey (1921-22 p. 90). 



1579. Bacto Malt Extract Agar (Synthetic) 

 (Dehydrated) 



Constituents: 



1. Distilled water 



2. Maltose, technical, Difco .... 12.75 g. 



3. Dextrin, Difco 2.75 g. 



4. Glycerol, C. P 2.35 g. 



5. Peptone, Bacto 0.78 g. 



6. Agar, Bacto 15.0 g. 



Preparation: 



(1) Dissolve 33.63 g. of Bacto Malt Ex- 

 tract Agar (Synthetic), (Dehydrated) 

 in 1000.0 cc. water by boiling or better 

 autoclaving. 

 Sterilization: Sterilize in the usual manner. 

 Use: Cultivation of yeasts and molds. 

 Reference: Digestive Ferments Co. (1925 

 p. 17). 



1580. Harvey's Glucose Glycerol Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone 15.0 g. 



3. Glucose 10.0 g. 



4. Glycerol 5.9 g. 



5. NaCl 5.0 g. 



6. Agar 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Do not adjust the reaction. 

 Sterilization: Not specified. 



Use: Cultivation of hyphomycetes. Klim- 

 mer cultivated favus and trichophyton 

 on a similar medium. 



Variants: Klimmer used 10.0 to 20.0 g. 

 peptone and 20.0 g. of NaCl. He cred- 

 ited Plaust with having described this 

 medium previously. 



References: Harvey (1921-22 p. 101), Klim- 

 mer (1923 p. 228). 



1581. Beijerinck's Glucose Peptone Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 20.0 g. 



