456 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Glucose 50.0 g. 



4. Peptone 20.0 g. 



5. KH2PO4 0.5 g. 



6. Ferric ammonium citrate... 0.1 g. 



7. Chalk 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, and 7 in 1. 



(2) Pour into plates. 

 Sterilization: Not specified. 



Use: To study pigment production by 

 vinegar bacteria. Author reported that 

 the medium was blackened. 



Reference: Beijerinck (1911 p. 172). 



1582. Sarbouraud's Glucose Peptone Agar 



(Serena) 



Constituents : 



1. Water 1000.0 cc. 



2. Glucose 40.0 g. 



3. Agar 18 g. 



4. Peptone 10.0 g. 



5. Tartaric acid 3.0 to 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Oidium suis. 

 Reference: Serena (1913 p. 280). 



1583. Chantemesse's Phenol Peptone Agar 



(Bezanfon) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone 30.0 g. 



3. Lactose 20.0 g. 



4. Agar 20.0 g. 



5. Phenol 



6. Litmus 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) When ready for use add 4 drops of a 

 3.0% aqueous phenol solution and 1.0 

 cc. of litmus solution to each 10.0 cc. 

 sterile (1). 



Use: Differentiation of colon-typhoid 

 group. Bezanfon reported that the colon 

 colonies were red, typhoid colonies color- 

 less. 



Variants: Harvey prepared the medium as 

 did Bezangon but added just before use, 

 0.05 cc. of a 5.0% phenol solution and 

 100.0 cc. of litmus solution to the entire 

 lot of sterile medium. The litmus 

 solution was prepared as follows: 

 (1) Grind up litmus in a mortar. 



(2) Add 5 volumes 90.0% alcohol. 



(3) Boil on a water bath. 



(4) Decant the supernatant fluid. 



(5) Add 6 parts distilled water to the 

 residue. 



(6) Boil. 



(7) Allow to cool. 



(8) Divide into two portions. 



(9) Render one portion slightly red with 

 dilute sulphuric acid. 



(10) Add to this reddened portion the 

 other untreated portion little by 

 little until the mixture becomes blue 

 again. 



(11) Filter thru paper when cool. 



(12) Distribute into test tubes. 



(13) Sterilize at 110°C. 



(14) Keep for use. 



References: Bezangon (1920 p. 341), Harvey 

 (1921-22 p. 90). 



1584. Boekhout and de Vries' Maltose 



Peptone Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 5.0 g. 



3. Sodium tartrate 5.0 g. 



4. Maltose 5.0 g. 



5. Agar 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 0.0, 2.0 or 5.0 g. of lactic acid or 

 0.0, 1.0, 2.0 or 5.0 g. NaOH to obtain a 

 neutral acid or alkaline reaction. 



Sterilization: Not specified. 



Use: Cultivation of Bacillus fuchsinus. 

 Author reported that the colonies were 

 first red and then a metallic sheen de- 

 veloped. Organism grew only on a 

 neutral or slightly alkaline medium. 



Reference: Boekhout and de Vries (1898 

 p. 500). 



1585. Cheyney's Maltose Peptone Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar (2.0%) 20.0 g. 



3. Maltose (1.0%) 10.0 g. 



4. Peptone (1.0%) 10.0 g. 



5. Lactic acid 

 Preparation: 



(1) Dissolve 2.0% agar in distilled water. 



(2) Filter thru a thin layer of cotton. 



(3) Add 1.0% maltose and 1.0% peptone. 



