CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



457 



(4) Make up to exactly 2.0% acidity by 

 the addition of lactic acid. 



(5) Tube. 



Sterilization: Autoclave for 10 minutes at 



15 pounds pressure. 

 Use: Cultivation of organisms found in 



canned food. 

 Reference: Cheyney (1919 p. 183). 



1586. Sabouraud's Glycerol Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2 Peptone 20.0 g. 



3. Glycerol 20.0 g. 



4. Acetic acid (glacial) 5 drops 



5. Agar 13.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of Seborrheaie's bacilli 



(Bacillus acne). 

 Reference: Sabouraud (1897 p. 144). 



1587. Robinson and Rettger's Glycerol 

 Opsine Citrate Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Opsine 20.0 g. 



3. NaCl 5 0g. 



4. KH2PO4 5 0g. 



5. Sodium citrate 2.0 g. 



6. MgS04 2 g. 



7. Glucose 5.0 g. 



8. Glycerol 60.0 g. 



9. Agar 15 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in 1. 



(2) Medium adjusted to very faintly 

 alkaline to litmus. 



Sterilization: Sterilize at 12 pounds for 15 

 minutes. 



Use: General culture media. Author re- 

 ported that the medium supported the 

 growth of some pathogenic organisms. 



Reference: Robinson and Rettger (1918 pp. 

 214, 215). 



1588. Botelho's Lacto-phenol Peptone Agar 



Constituents: 



1. Distilled water 



2. Lactic acid 



3. Phenol (snowy) 



4. Glycerol 



5. Cotton blue ("Poirier C^B") 



6. Sugar (white crystallized) 



7. NaCl 5.0 g. 



8. Peptone (Chapoteaut) 10.0 g. 



9. Agar 15.0 g. 



Preparation : 



(1) Prepare a lactophenol solution by 

 mixing the following materials: 

 Distilled water 1 part, glycerol 2 

 parts, pure lactic acid 1 part, snowy 

 phenol 1 part. 



(2) Add 0.5 centigrams of cotton blue 

 "Poirier C^B" to 30.0 g. of (1). 



(3) Mix (2) and dilute to i with distilled 

 water. 



(4) Mix 200.0 g. of crystalline white 

 sugar in 100.0 cc. of cold water. 



(5) Boil (4) and stir until solution is 

 complete. 



(6) Filter thru flannel. 



(7) Dissolve 15.0 g. agar, 10.0 g. Chapo- 

 teaut's peptone, and 5.0 g. of NaCI 

 in 1000.0 cc. of water by boiling. 



(8) Filter, but do not neutralize. 



(9) Measure 100.0 cc. of melted sterile 

 (8) into a balloon flask. 



(10) Add 2.0 cc. of (3) and 10.0 cc. of (9) 

 and mix well. 



(11) Boil. 



(12) Add 7.0 cc. of a 2.0% NaOH solution 

 in distilled water. This completely 

 decolorizes the agar. 



(13) Distribute in 3.0 cc. lots into small 

 sterile Wassermann tubes. 



Sterilization: Sterilize (8) in the autoclave. 



Use: Isolation of members of colon typhoid 

 group. Author reported that members 

 of the colon-typhoid group color the 

 medium blue. 



Reference: Botelho (1917 p. 436). 



1589. Sullivan's Ammonium Lactate 

 Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Peptone, Witte's 15.0 g. 



3. NaCl 3.0 g. 



4. K2HPO4 1.5 g. 



5. MgS04 0.5 g. 



6. Fe2(S04)3 trace 



7. CaCls trace 



8. Ammonium lactate 0.5 g. 



9. Glycerol 20.0 g. 



10. Agar 10.0 g. 



