458 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8. 9 and 10 in 1. 



Sterilization: Method not given. 



Use: General culture medium. Author re- 

 ported that putrefactive, phosphorescent, 

 chromogenic and pathogenic varieties 

 developed. If the salts CaCh, Fe2(S04)3 

 and NaCl be omitted number of organisms 

 grown was not markedly decreased. 



Reference: Sullivan (1905-06 p. 113). 



1590. Lipman and Brown's Nitrate Peptone 

 Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Dextrose 10.0 g. 



3. K2HPO4 0.5 g. 



4. MgS04 0.2 g. 



5. Agar 20.0 g. 



6. Peptone 0.1 to 0.5 g. 



7. KNO3 0.05 to 0.5 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) To (1) containing 0.5 g. peptone, add 

 0.05 to 0.1, 0.2, or 0.5 g. KNO3. 



Sterilization: Method not specified. 



Use: Determination of bacterial count of 

 soil. Author reported that different soils 

 showed different results with the same 

 peptone and nitrate concentration. 



Variants : 



(a) See medium 1549. 



(b) Harvey used 0.06 g. KNO3. 

 References: Lipman and Brown (1910 p. 



451), Harvey (1921-22 p. 105). 



1591. Beijerinck's Ferric Ammonium 

 Citrate Peptone Agar (Janke) 



Constituents : 



1. Water 



2. Agar 



3. Glucose 



4. Peptone 



5. KH2PO4 



1000.0 cc 



2.0 g 



5.0 g, 



2.0 g 



0.05 g 



6. Ferric ammonium citrate . . 0.01 g 



7. Chalk 2.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5, 6, and 7 in 1. 

 Sterilization: Method not given. 

 Use: Cultivation of acetic acid bacteria in 



beers. 

 Reference: Janke (1916 p. 6). 



1592. Vierling's Nitrate Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. CaCl2 1.0 g. 



4. MgS04 0.1 g. 



5. FeCU trace 



6. NaCl trace 



7. Agar (2.0%) 20.0 g. 



8. Peptone (1.0%) 10.0 g. 



9. Dextrose (1.0%) 10.0 g. 



10. KNO3 (0.75%) 7.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1000.0 cc. 

 water. 



(2) Dissolve 2.0% agar, 1.0%, peptone, 

 1.0% de.xtrose and 1.0% KNO3 in 

 100.0 cc. of (1). 



(3) Pour in petri dishes. 

 Sterilization: Method not given. 



Use: To study nitrate reduction by myco- 

 bacteria. To test nitrate production, 

 pour 2.0% KI solution over the plates 

 which have been acidified with dilute 

 acetic acid, li KNO2 is formed, the 

 iodine will be freed, coloring the starch 

 blue. The addition of starch to the 

 medium is not specified however. 



References: Vierling (1920 p. 201). 



1593. Heinemann's Asparagin Peptone Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 15 g. 



3. Asparagin 5.0 g. 



4. K2HPO4 2.0 g. 



5. Na2HP04 2.0 g. 



6. MgS04 2 0g. 



7. CaCl2 2.0 g. 



8. Ammonium lactate 2.0 g. 



9. Peptone 10.0 g. 



10. Starch (washed) 30.0 g. 



Preparation : 



(1) Dissolve agar in 600.0 cc. of water by 

 heating. 



(2) Dissolve 3, 4, 5, 6, 7 and 8 in 200.0 cc. 

 of water. (A fine precipitate is 

 formed) . 



(3) Add (2) to hot (1). 



(4) Add 9. 



(5) Adjust to neutral with phenol- 

 phthalein. 



