CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



461 



(4) Dissolve the ingredients by bubbling 

 live steam thru the mixture. 



(5) Weigh out 1.0 g. aesculin and 0.5 g. 

 ferric citrate and dissolve in a second 

 flask containing 100.0 cc. distilled 

 water. 



(6) Mix the contents of the two flasks — 

 adjust the weight to the calculated 

 medium figure (in this case 1031. 5g.) 

 by the addition of distilled water at 

 100°C. 



(7) Clarify with egg and filter. 



(8) Tube and sterilize as for nutrient 

 agar. 



References: Harrison and VanderLeck 

 (1909 p. 549, 607), Committee Am. Public 

 Health Asso. (1909 p. 286), Harvey (1921- 

 22 p. 89), Levine (1921 p. 117),Heinemann 

 (1922 p. 33). 



1596. Harvey's Brilliant Green Bile Salt 

 Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Sodium taurocholate 5.0 g. 



3. Peptone 30.0 g. 



4. NaCl 5.0 g. 



5. Lactose 10.0 g. 



6. Powdered agar 30.0 g. 



7. 1-1000 Brilliant-green 20.0 cc. 



8. Picric acid (1.0%) 20.0 cc. 



Preparation : 



<1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1000.0 



cc. of water. 

 (2) Reaction to be 1.5% acid to phenol- 

 phthalein. 

 Sterilization: Method not given. 

 Use: Enrichment medium for colon- 

 typhoid group. 

 Reference: Harvey (1921-22 p. 91). 



SUBGROUP II-C. SECTION 4 



Basal or complete media containing agar 

 and peptone (or other commercial digest) 

 together with additional constituents of 

 unknown chemical composition of plant or 

 soil origin. 



Ai. Containing yeast or yeast derivatives. 

 Bi. All additional constituents of known 



chemical composition. 



Gassner's Yeast E.xtract Peptone 

 Agar 1597 



Cohen and Clark's Yeast E.xtract 

 Peptone Agar 1598 



Ickert's Yeast Infusion Peptone 



Agar 1599 



Sherman's Yeast Extract Peptone 



Agar 1600 



Bj. Containing additional constituents of 

 unknown chemical composition. 

 Rivers and Kohn's Basal Yeast Ex- 

 tract Peptone Agar 1601 



Sturtevant's Egg Yolk Yeast Agar . 1602 

 A2. Containing bacterial or fungus de- 

 rivatives. 



Savini and Savini-Castano's Hemo- 

 globin Bacterial Extract Agar. . . . 1603 

 Mankowski's Fungus Infusion Pep- 

 tone Agar 1604 



A3. Containing derivatives of plants other 



than Ai and A2 above. 

 Bi. Plant juices or tissues (not infusions) 

 employed. 



Owens' Cane Juice Peptone Agar. . . 1605 

 Plaisance and Hammer's Corn Juice 



Peptone Agar 1606 



Peglion's Grape Must Peptone 



Agar 1607 



Harvey's Banana Agar 1608 



B2. Plant infusions, extracts, etc., em- 

 ployed. 

 Ci. Grain derivatives used. 



Meacham et al. Malt Extract Agar. . 1609 

 Marpmann's Flour Casein Agar .... 1610 

 Williams and Povitzky's Flour Pep- 

 tone Agar 1611 



Otabe's Wheat Peptone Agar 1612 



Dawson's Flour Peptone Agar 1613 



Plaisance and Hammer's Stover 



Infusion Peptone Agar 1614 



C2. Potato derivatives used. 

 De Gaetano's Potato Extract Agar . . 1615 

 Jochmann's Potato Bouillon Agar.. 1616 

 Nicholle and Alilaire's Potato In- 

 fusion Agar 1617 



Shiga et al. Potato Blood Agar 1618 



Gaehtgen's Potato Peptone Agar. . . 1619 

 C3. Derivatives of legumes used. 



Matzuschita's Pea Bile Agar 1620 



Tanner's Pea Extract Tryp. Agar... 1621 



Behrens' Pea Blood Agar 1622 



De Rossi's V. Faba Infusion Pep- 

 tone Agar 1623 



C4. Miscellaneous plant derivatives used. 



Owens' Molasses Peptone Agar 1624 



Seiffert and Bamberger's Chloro- 

 phyll Bouillon Agar 1625 



Dawson's Edestin Peptone Agar. . . . 1626 



