462 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



A4. Containing soil or its derivatives. 

 Temple's Soil Infusion Peptone 

 Agar 1627 



1597. Gassner's Yeast Extract Peptone 



Agar 



Constituents : 



1. Water 900.0 cc. 



2 Yeast extract water 900.0 cc. 



3. Agar 30.0 g. 



4. NaCl 5.0 g. 



5. Peptone 20.0 g. 



Preparation: 



(1) Exact method of yeast extract water 

 not given. 



(2) Mix 1 and (1). 



(3) Dissolve 3 and 4. 



(4) Adjust to slightly alkaline to litmus. 



(5) Dissolve 5 in (4). 



(6) To each 80.0 cc. of (5) add 20.0 cc.of 

 a 0.5% solution of water blue and 0.5, 

 0.1 or 1.0% dextrose. 



Sterilization: Not specified. 



Use: To study nitrogen requirements of 



colon-typhoid and dysentery group. 

 Variants: The author omitted the glucose. 

 Reference: Gassner (1917-18 p. 260). 



1598. Cohen and Clark's Yeast Extract 



Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Yeast, Brewer's 10.0 g. 



3. Peptone, Difco 10 g. 



4. K2HPO4 5.0 g. 



5. Agar (amount not given) 



6. Glucose 10.0 g. 



Preparation: 



(1) Dissolve 5 in an extract of 10.0 g. of 

 Brewer's yeast in 1000.0 cc. water 

 containing 3, 4 and 6. 



(2) Filter. 



(3) Adjust with NaOH and HCl to desired 

 pH. 



Sterilization: Method not given. 

 Use: To study growth of B. vulgaricvs and 

 B. coll at different H-ion concentrations. 

 Reference: Cohen and Clark (1919 p. 421). 



1599. Ickert's Yeast Infusion Peptone Agar 



Same as medium 695 but solidified by the 

 addition of agar. 



1600. Sherman's Yeast Extract Peptone 

 Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 20.0 g. 



3. Yeast 10.0 g. 



4. Lactic acid (as sodimn 

 lactate) 10.0 g. 



5. Agar 1.5 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Details of method of preparation not 

 given. 



Sterilization: Method not given. 



Use: Isolation of organisms causing flavors 



in cheese. 

 Reference: Sherman (1921 p. 388). 



1601. Rivers and Kohn's Basal Yeast 

 Extract Peptone Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Peptone 20 g. 



3. Agar agar 15.0 g. 



4. NaCl 5 0g. 



5. Blood clot extract 



6. Yeast extract 



7. Brom Cresol Purple (25 0% 

 alcoholic soln.) 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 by boiling 



(2) Adjust the reaction to pH = 7.4. 



(3) Filter. 



(4) To 15.0 cc. of sterile (3) add 20.0 cc. of 

 sterile blood clot extract (method 

 of preparation not given) and 15 to 

 20.0 cc. of yeast extract (preparation 

 not given). 



(5) Add 10.0 cc. of a 10.0% solution 

 of any one of the added nutrients, and 

 25.0% alcoholic solution of brom 

 cresol purple to give a good color. 



(6) Incubate to test sterility. 

 Sterilization: Sterilize (3) and the blood 



extract in autoclave. Sterilization of 



other materials not given. 

 Use: To study fermentation ability of 



influenza bacillus producing meningitis. 

 Added nutrients: The authors added 10.0 



cc. of a 10.0% solution of any desired 



carbohydrate, alcohol, etc., to each 100.0 



cc. of medium. 

 Reference: Rivers and Kohn (1921 p. 381). 



