464 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Differentiation of typhoid bacilli and 

 Bacterium coli communis. Author re- 

 ported that Bacterium coli grew in a 

 silver white, solid and dry film. Typhoid 

 bacilli grew slower, appeared as trans- 

 parent, shining, damp strips. When 

 fuchsin indigo carmine be added typhoid 

 bacilli colored the blue medium red, 

 Bacterium coli communis gave a bluish 

 green color and finally completely de- 

 colorized the medium. 



Variants : 



(a) The author prepared a medium con- 

 taining fuchsin and indigo carmine 

 by continuing from step (4) above as 

 follows: 



(5) Prepare a saturated solution of 

 acid fuchsin in a 1.0% KOH solu- 

 tion (acid fuchsin may be added 

 to a 1.0% KOH solution until a 

 dark black brown color is reached). 



(6) Prepare a watery saturated solu- 

 tion of indigo carmine. 



(7) Add 2.0 cc. of (2) and 1.0 cc. of 

 (3) to 22.0 cc. of distilled water. 

 This solution is dark blue and 

 reaction slightly alkaline. 



(8) Add (7) drop by drop to (4) until 

 the agar is colored blue and then 

 violet blue. 



(9) Distribute into test tubes. 



(10) Add to each test tube a drop of 

 watery saturated solution of 

 indigo carmine. 



(b) Wiegert solidified medium 701 by the 

 addition of agar. 



References: Mankowski (1900 p. 23), 

 Wiegert (1921-22 p. 110). 



1605. Owens' Cane Juice Peptone Agar 



Constituents: 



1. Cane juice 1000.0 cc. 



2. Agar (2.0%) 20.0 g. 



3. Peptone (1.0%) 10.0 g. 



Preparation : 



(1) Heat fresh raw cane juice. 



(2) Filter thru cotton. 



(3) Add 1.0% peptone (peptone may be 

 omitted). 



(4) Solidify by the addition of 2.0% agar. 

 Sterilization: Not specified. 



Use: Bacterial count of cane sugar 



products. 

 Reference: Owen (1914 p. 337). 



1606. Plaisance and Hammer's Corn Juice 



Peptone Agar 



Constituents : 



1. Corn juice 1000.0 cc. 



2. Agar 15.0 g. 



3. Peptone 10.0 g. 



Preparation : 



(1) Obtain corn juice by pressing green 

 corn. 



(2) Dissolve 2 and 3 in (1). 



(3) Adjustment of reaction not specified. 

 Sterilization: Not specified. 



Use: Study mannitol producing organisms 



from silage. 

 Reference: Plaisance and Hammer (1921 p. 



432). 



1607. Peglion's Grape Must Peptone Agar 



Constituents : 



1. Grape must 500.0 cc. 



2. Peptone 10.0 g. 



3. Ammonium phosphate 2.0 g. 



4. Glycerol 10.0 cc. 



5. Agar 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Pour into petri dishes or plates. 

 Sterilization: Not specified. 



Use: Cultivation of Saccharomyces ellip- 



soideus. 

 Reference: Peglion (1898 p. 477). 



1608. Harvey's Banana Agar 



Constituents: 



1. Agar (nutrient) 1000.0 cc. 



2. Banana (10.0%) 100.0 g. 



Preparation : 



(1) Use banana in the form of cut cylinder 

 or as 10.0% pulp incorporated with 

 nutrient agar. (Medium 779 variant 

 (bb) solidified by the addition of 

 agar.) 



Sterilization: Not specified. 



Use: General culture medium. 



Reference: Harvey (1921-22 p. 119). 



1609. Meacham et al. Malt Extract Agar 



Constituents : 



1. Malt extract (2.5%) agar. . . . 1000.0 cc. 



2. K2HPO4 M/50 



3. Acetic acid N/50 

 Preparation : 



(1) Preparation of malt extract agar not 

 given. 



