CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



465 



(2) Dissolve 2 and 4 in (1). 



(3) Reaction about pH = 5.7. 

 Sterilization: Method not given. 



Use: To study phosphate-phthalate and 

 phosphate-acetate as buffers for cultiva- 

 tion of E. parasitica (Murr). Author 

 reported that acid phthalate gave best 

 growth of E. parasitica. 



Variants : Author used M/50 acid potassium 

 phthalate instead of acetic acid. 



Reference: Meacham, Hopfield and Acree 

 (1920 p. 305). 



1610. Marpmann's Flour Casein Agar 

 Constituents: 



1. Bouillon 1000.0 cc 



2. Glycerol 30.0 g 



3. Casein, dissolved 50.0 g 



4. Sodium phosphate 30 g, 



5. Rye flour 50.0 g 



6. Gelatin 40.0 g 



7- Agar 3.0 g 



Preparation : 



(1) Method of preparation or exact 

 composition of bouillon not given. 



(2) Dissolve 2, 3, 4, 5, 6 and 7 in (1). 



(3) Adjustment of reaction not given. 



(4) Distribute into flat bottomed flasks. 

 Sterilization: Method not given. 



Use: Cultivation of tubercle bacilli. 

 Reference: Marpmann (1903 p. 636). 



1611. Williams and Povitzky's Flour 



Peptone Agar 

 Constituents : 



1. Water (tap) 1300 cc. 



2. Wheat 1.0 lb. 



3. Diastase 0.5 g. 



4. NaCl 5.0 g. 



5. Peptone 10.0 g. 



6. Agar (1.5%) 15.0 g.' 



7. Gelatin (2.0%) 20.0 g. 



Preparation : 



(1) Roast whole wheat (with or without 

 the husk) in pan until brown. 



(2) To one pound roasted wheat add 

 1300.0 cc. tap water. 



(3) Steam in Arnold for one-half hour. 



(4) Strain thru fine wire strainer. 



(5) Make up to 1000.0 cc. with tap water. 



(6) Cool to 35°C. and add Taka diastase 

 or ordinary diastase, 0.5 gm. and 

 shake well. Keep flask at 30-40 °C. 

 for half hour. 



(7) Add sodium chloride, 5 g. and pep- 

 tone 10.0 g. 



(8) Boil. Reaction adjusted to pH-7.4. 



(9) Add agar 1.5% and autoclave at 15 

 pounds pressure for half hour. 



(10) On removal from autoclave add 

 gelatin 2.0%. Adjust reaction. 



(11) Cool down to 45°C. and clarify with 

 white of egg. 



(12) Heat in the Arnold steamer 45 

 minutes. Correct reaction to pH-7.6. 

 This will give desired end reaction 

 (pH-7.4), after autoclaving. 



(13) Filter thru thin cotton (3 times) and 

 tube. 



Sterilization: Sterilize the tubes of agar for 

 30 minutes at 15 pounds pressure. 



Use: Cultivation of B. influenzae. Author 

 reported that growth of the influenza 

 organism was obtained when mixed with 

 other organisms. 



Reference: Williams and Povitzky (1920- 

 21 p. 407). 



1612. Otabe's Wheat Peptone Agar 



Solidify medium 707 by the addition of 1.5 

 to 2.0% agar. 



1613. Dawson's Flour Peptone Agar 

 Constituents : 



1. Water 1000.0 cc. 



2. Peptone 2.5 g. 



3. Flour protein 10.0 g. 



4. Agar 20.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Reaction to be alkaline. 

 Sterilization: Method not given. 



Use: Culture medium to study variation of 



B. coli. 

 Reference: Dawson (1919 p. 142). 



1614. Plaisance and Hammer's Stover 

 Infusion Peptone Agar 

 Constituents : 



1. Stover juice 1000.0 cc. 



2. Agar 15.0 g. 



3. Peptone 10.0 g. 



4. Sucrose 5.0 g. 



Preparation: 



(1) Prepare stover juice by soaking corn 

 stover in water for 12 hours and then 

 pressing. 



(2) Dissolve 2, 3 and 4 in (1). 



