466 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(3) May be cleared with egg. 

 Sterilization : Not specified. 

 Use: Study mannitol producing organisms 



from silage. Authors reported this 



medium to be inferior to 1606. 

 Reference: Plaisance and Hammer (1921 



p. 432). 



1615. De Gaetano's Potato Extract Agar 



Constituents : 



1. Potato infusion 1000.0 cc. 



2. Meat extract (Lie- 

 big's) 20.0 g. 



3. Peptone 10.0 g. 



4. Agar 20.0 to 30.0 g. 



5. Tartaric acid 



6. Glucose 

 Preparation : 



(1) Preparation of potato infusion not 

 given. 



(2) Dissolve 2, 3, and 4 in (1) by boiling. 



(3) Filter. 



(4) Distribute in 6.0 cc. lots in tubes. 



(5) To each tube add 5.0 to 6.0 drops of 

 10.0 to 15 0% tartaric acid solution 

 and 5.0 to 6.0 drops of a 50.0% glucose 

 solution. 



Sterilization: Method not given. 



Use: Cultivation of Saccharomyces 



septicus. 

 References: De Gaetano (1897 ^200), 



Kamen (1899 p. 833). 



1616. Jochmann's Potato Bouillon Agar 



Constituents : 



1. Bouillon 200 cc. 



2. Potato 600.0 g. 



3. Agar 3.75 g. 



Preparation : 



(1) Grate 600.0 g. of potatoes and allow 

 to stand in a glass flask at about 

 15°C. for 12 hours. 



(2) Press the juice thru a filter cloth using 

 the hands to expell all the juice. 



(3) To 300.0 g. of (2) add 200.0 g. of 

 weakly alkaline bouillon (exact compo- 

 sition or method of preparation not 

 given). 



(4) Dissolve completely 3.75 g. finely 

 divided agar in (3) using the 

 autoclave. 



(5) The reaction is slightly acid. 

 Sterilization: Not specified. 



Use: Diagnosis of typhoid fever. Author 

 reported that typhoid colonies after 12 

 hours were typical, small, silver gray, 

 glistening, fine fibered; coli colonies 

 larger, light yellow and mostly all round. 

 Medium was dark yellow or dark brown 

 in color. 



Reference: Jochmann (1902 p. 461). 



1617. KicoUe and Alilaire's Potato Infusion 

 Agar 



Constituents : 



1. Water 2000.0 cc. 



2. Potato 500.0 g. 



3. Beef 500.0 g. 



4. Peptone (Chapoteaut) 30.0 g. 



5. NaCl 10.0 g. 



6. Glycerol 20.0 g. 



7. Agar 60.0 g. 



Preparation : 



(1) Extract 500.0 g. of finely chopped beef 

 with one liter of water over night. 



(2) Extract 500.0 g. of potatoes cut in 

 large pieces in a second liter of water 

 over night. 



(3) Mix (1) and (2). 



(4) Dissolve 4, 5, 6 and 7 in (3). 



(5) Proceed in the ordinary manner for 

 the rest of the preparation for nu- 

 trient agar (Method not given) . 



Sterilization: Not specified. 



Use : Mass cultivation of B. encapsulas and 



Cholera vibrio. 

 Variants: Lubinski solidified medium 873 



by the addition of I'.O to 1.5% agar. 

 References: NicoUe and Alilaire (1909 p. 



548), Lubinski (1895 p. 126). 



1618. Shiga et al Potato Blood Agar 



Constituents: 



1 . Glvcerol (4.0% soln) 250 cc. 



2. Potato 125.0 g. 



3. Meat water 750.0 cc. 



4. Agar 20.0 g. 



5. Peptone 100 g. 



6. NaCl 5.0 g. 



7. Blood (beef or horse) 4000.0 cc. 



Preparation : 



(1) Peel 500.0 g. of potatoes and cut into 

 small pieces. 



(2) Add (1) to 1000.0 cc. of 4.0% glycerol 

 water. 



(3) Boil for 30 minutes in the autoclave. 



(4) Filter thru gauze. 



