CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



467 



(5) Method of preparation of meat water 

 not given. 



(6) Mix 250.0 cc. of (4) and 750.0 cc. of (5). 



(7) Add 20.0 g. of agar. 



(8) Boil in the autoclave for one hour. 



(9) Make slightly alkaline. 



(10) Filter. 



(11) Add 1.0% Witte's peptone and 0.5% 

 NaCl. 



(12) Distribute into tubes. 



(13) Melt and cool to 50°C. when ready 

 for use. 



(14) Add agar to beef or horse blood in 

 the ration of 1:4. 



(15) Tube and slant or pour into petri 

 dishes. 



(16) After solidification heat the agar 

 at 56°C. for 30 minutes. 



Sterilization: Not specified. 



Use : Cultivation of whooping cough bacilli 



and influenza bacilli. 

 Reference: Shiga, Imai and Eguchi (1913 



p. 104). 



1619. Gaehtgen's Potato Peptone Agar 



Constituents: 



1. Water 1000.0 cc, 



2. Potato 500.0 g. 



3. Agar 



4. Peptone 



5. NaCl 

 Preparation : 



(1) Carefully wash and peel 500.0 g. 

 potatoes. 



(2) Grind the potatoes fine by means of a 

 porcelain mortar. This is to be 

 carried out under the water as far as 

 possible. 



(3) Boil for one hour. 



(4) Dissolve 3, 4 and 5 (amounts not 

 given) in the paste. 



(5) Add soda to obtain the desired 

 reaction. 



Sterilization: Method not given. 



Use: Inexpensive culture Medium. Author 

 reported that the medium was not trans- 

 parent. 



Reference: Gaehtgens (1916 p. 47). 



1620. Matzuschita's Pea Bile Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Liver, ox 500.0 g. 



3. Pea flour 30.0 g. 



4. Peptone (0.7%) 7.0 g. 



5. NaCl (0.5%) 5.0 g. 



6. Bile, ox 600.0 g. 



7. Agar (2.0%) 32.0 g. 



Preparation : 



(1) Boil 500.0 g. of finely chopped liver 

 with 30.0 g. of pea flour and one liter 

 of water. 



(2) Allow to cool and add 0.5% peptone, 

 0.5% NaCl and 0.02% HCl. 



(3) Mix thoroly and allow to stand for 3 

 hours at 37°C. 



(4) Add 600.0 g. of ox bile and allow to 

 stand another 3 hours at 37°C. 



(5) Proceed as in the preparation of 

 regular nutrient agar by boiling, 

 filtering, adding 2.0% agar and 

 filtering again. 



(6) Reaction is slightly acid. 

 Sterilization: Method not given. 

 Use: Cultivation of intestinal bacteria. 

 Reference: Matzuschita (1901-02 p. 214). 



1621. Tanner's Pea Extract Tryp. Agar 



Constituents : 



1. Medium 1116 1000.0 cc. 



2 Agar (2.0%) 20.0 g. 



3. CaClo 0.125 g. 



4. Pea extract (5.0%) 50.0 cc. 



Preparation : 



(1) Add 2.0% agar fiber and 0.125 g. of 

 CaCl2 to 1000.0 cc. of trypsinized 

 broth, medium 1116. 



(2) Autoclave at 118°C. for 45 minutes to 

 dissolve the agar. 



(3) Mix together in a sauce pan. 



(4) Titrate with N/10 KOH to give an 

 absolutely neutral reaction. 



(5) Cool to 60°C. 



(6) Add the whites of two eggs beaten 

 up with the crushed shells. 



(7) Autoclave again at 118°C. for 75 

 minutes (or in the steamer for two 

 hours). 



(8) Filter. 



(9) Add 5.0% sterile pea extract (prepara- 

 tion not given). 



Sterilization: Sterilize in the ordinary way 



(method not given). 

 Use: General culture medium. 

 Reference: Tanner (1919 p. 49). 



