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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



1622. Behrens' Pea Blood Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peas 10.0 g. 



3. Peptone 20.0 g. 



4. NaCl 5.0 g. 



5. CaClj 0.1 g. 



6. N/1 NajCO, soln 10.0 cc. 



7. Agar 20.0 g. 



8. Blood (rabbit) 2000.0 cc. 



Preparation: 



(1) Boil 10.0 g. peas in 1000.0 cc. distilled 

 water to obtain extract. Length of 

 boiling not specified. 



(2) Strain, boil the extract and filter. 



(3) Dialyze the filtrate in a large collo- 

 dium sac against running distilled 

 water for 24 to 48 hours. 



(4) Dilute (3) to 1000.0 cc. 



(5) Dissolve 3, 4, 5, 6 and 7 in (4). 



(6) Distribute in test tubes in 1.0 cc. lots. 



(7) Shortly before use the desired number 

 of sterile agar tubes are melted in 

 the water bath, cooled to 60°C. and 

 two volumes of defibrinated rabbit's 

 blood are added. 



(8) Mix well and solidify in slanting 

 position. 



Sterilization: Sterilize by autoclaving at 

 105 to 108°C. for 15 minutes. 



Use: Special culture medium for Trypano- 

 soma Brucei. 



Variants : The author used beans instead of 

 peas. 



Reference: Behrens (1914 p. 28). 



1623. De'Rossi's V.Faba Infusion Peptone 

 Agar 



Constituents : 



1. Water 1000.0 cc. 



2. V. Faba leaves 100.0 g. 



3. Agar (1.5%) 15.0 g. 



4. NaCl 5.0 g. 



5. Sucrose (2.0%) 20.0 g. 



6. Peptone (1.0%) 10.0 g. 



Preparation : 



(1) Boil 8 to 10 parts water with Vicia 

 Faba leaves. 



(2) Filter and add 0.5% NaCl, 2.0% 

 sucrose and 1.0% peptone. (NaCl, 

 sucrose and peptone may be omitted.) 



(3) Continue in the same manner as in 

 the preparation of nutrient agar. 



(4) The reaction is slightly acid or 

 alkaline. 

 Sterilization: Not specified. 

 Use: Cultivation of bacteria found in the 



nodules of leguminous plants. 

 Variants: The author used 2.0% glucose 



instead of sucrose. 

 Reference: de'Rossi (1907 p. 301). 



1624. Owen's Molasses Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Molasses (Final 75 Brix) . . . 160.0 g. 



3. Agar 20.0 g. 



4. Peptone 10.0 g. 



Preparation : 



(1) Dissolve 160.0 g. of final molasses, 

 75 Brix, 20.0 g. agar and 10.0 g. pep- 

 tone in 1000.0 cc. water. 

 Sterilization: Not specified. 

 Use : Bacterial counts of organisms found in 



corn juice and cane sugar products. 

 Reference: Owen (1914 p. 338). 



1625. Seiffert and Bamberger's Chlorophyll 

 Bouillon Agar 



Constituents : 



1. Neutral agar 1000.0 cc. 



2. Chlorophyll solution ("So- 



lutio spirituosa," Merck)... 25.0 cc. 



3. Sucrose 



4. Dextrin 



5. Diamond fuchsin solution 



6. NasSOs 



7. Soda 

 Preparation : 



(1) Prepare 60.0 cc. of a 10.0% soda 

 solution using water free soda. 



(2) Add 25.0 cc. chlorophyll solution 

 (Solutio spirituose Merck) to (1). 



(3) Heat for one hour in the steamer. 



(4) Prepare a 20.0% sucrose solution. 



(5) Prepare a 20.0% dextrin solution. 



(6) Add 50.0 cc. sterile (4) and 50.0 cc. 

 sterile (5) to (3). 



(7) Mix with one liter sterile neutral 

 agar. 



(8) Prepare a diamond fuchsin add- 

 ing an excess of diamond fuchsin 

 to absolute alcohol and incubating 

 for 24 hours. Shake often. 



(9) Add 4.0 cc. of (8) to (7) when ready 

 for use. 



