CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



469 



(10) Add about 15.0 cc. of a 10.0% sterile 

 Na2S0j solution to decolorize (9). 



(11) Pour in sterile plates. 



(12) Dry the plates in the air. 

 Sterilization: Method of sterilization of 



(4), (5) or neutral agar not given. 



Use: Selective medium for cholera. Au- 

 thor reported that the cholera colonies 

 were red. Other organisms were inhibited. 



Reference: Seiffert and Bamberger (1916 p. 

 288). 



1626. Dawson's Edestin Peptone Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 5.0 g. 



3. Edestin 5.0 g. 



4. Agar 20.0 g. 



Preparation: 



(1) Dissolve 2, 3 and 4 in 1, 



(2) Reaction to be alkaline. 

 Sterilization: Method not given. 



Use: Culture medium to study variation of 



B. coll. 

 Reference: Dawson (1919 p. 142). 



1627. Temple's Soil Infusion Peptone Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Soil 100.0 g. 



3. K2HPO4 1.0 g. 



4. Peptone 10.0 g. 



5. Agar 15.0 g. 



Preparation: 



(1) Prepare a soil infusion by adding 

 1000.0 cc of water to 100.0 g. soil and 

 bringing to a boil. 



(2) Filter. 



(3) Dissolve 3, 4 and 5 in (2). 



(4) Adjust the reaction to +0.5. 

 Sterilization: Not specified. 



Use: Determination of bacterial counts 



from soil. 

 Variants: Brown suggested the use of 0.05 



g. peptone instead of 10.0 g. 

 References: Temple (1912 p. 205), Brown 



(1913 p. 499). 



SUBGROUP II-C. SECTION 5 



Basal or complete media containing agar 

 and peptone (or other commercial digest) 

 and at least one other organic material of 

 unknown composition. 



Ai. Animal cells and tissues or their deriva- 

 tives added. 



Rivers and Kohn's Basal Blood Cell 

 Peptone Agar 1628 



Krumwiede, Pratt and Grund's Egg 

 Peptone Agar 1629 



Weiss' Nahrstoff Heyden Gelatin 

 Agar 1630 



Deycke's Alkaline Albumin Gelatin 

 Agar 1631 



Deycke's Albuminate Glycerol 

 Agar :.... 1631a 



Krumwiede and Pratt's Gelatin 

 Serum Agar 1632 



Dunschmann's Bile Salt Peptone 

 Agar (Bezanyon) 1633 



Capaldi's Peptone Gelatin Agar 1634 



Smyth's Egg Trypsinized Peptone 



Agar 1635 



Aj. Animal fluids added. 

 Bi. Blood employed. 



Czaplewski's Blood Nahrstoff Hey- 

 den Agar (Klimmer) 1636 



Norris et al. Blood Peptone Agar. . . 1637 



Nicolle's Blood Peptone Agar 

 (Behrens) 1638 



Kelser's Blood Peptone Agar 1639 



Harvey's Blood Peptone Agar 1640 



Tildes' Blood Peptone Agar 1641 



Golowkoff's Blood Peptone Agar 

 (Uche) 1642 



Harvey's Blood Serum Peptone 

 Agar 1643 



Harvey's Litmus Blood Peptone 



Agar 1644 



B2. Serum or ascitic fluid employed. 



Tochtermann's Serum Peptone 

 Agar 1645 



Browning's Telluric Acid Serum 

 Agar (Wood) 1646 



Teague and Deibert's Serum Pep- 

 tone Agar 1647 



Emile-Weil's Pleuritic Serum Pep- 

 tone Agar 1648 



B3. Bile or its derivatives employed. 



Bacto MacConkey's Agar (De- 

 hydrated) 1649 



Jordan's Basal Peptone Bile Agar.. 1650 



Padlewsky's Bile Peptone Agar 

 (Bezan^on) 1651 



Northrup's Peptone Bile Agar 1652 



A3. Animal secretions, excretions or their 



derivatives added. 



