470 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Bacto Whey Agar (Dehydrated).. . 1653 

 Sabouraud and Noire's Basal Whey 

 Peptone Agar (Weil & Noire) .... 1654 



Tanner's Whey Peptone Agar 1655 



Committee A. P. H. A. Whey Pep- 

 tone Agar 1656 



Huss' Whey Peptone Agar 1657 



Heller's Peptone Urine Agar 1658 



Loeffler's Malachite Green Nutrose 



Peptone Agar 1659 



Raskin's Whey Peptone Agar 1660 



1628. Rivers and Kohn's Basic Blood Cell 



Peptone Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone (Fairchild) 20.0 g. 



3. NaCl 5.0 g. 



4. Agar 15.0 g. 



5. Red Blood Cells 10.0 cc. 



6. Brom cresol purple (25.0% 

 ale. soln.) 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 by boiling. 



(2) Titrate to pH = 7.4. 



(3) Filter and distribute in 100.0 cc. lots. 



(4) At the time the agar is taken from the 

 autoclave while still at 95 °C. add 1.0 

 cc. of washed red blood cells, 10.0 cc. 

 of a 10.0% solution of one of the added 

 nutrients and enough 25.0% alcoholic 

 solution of brom cresol purple to each 

 100.0 cc. to give a good color. 



(5) Tube while still warm. 



(6) Incubate to test sterility. 



Sterilization: Sterilize (3) in the autoclave. 



Use: To study fermentation. Authors re- 

 ported that the medium had a dirty look- 

 ing appearance but this did not interfere 

 with the results. 



Added nutrients: The authors added 10.0 

 cc. of a 10.0% solution of one of the 

 following to each 100.0 cc. of agar: 

 glucose fructose 



galactose maltose 



xylose 

 Reference: Rivers & Kohn (1921 p. 481). 



1629. Krumwiede, Pratt and Grund's Egg 



Peptone Agar 



Constituents : 



1. Water 850.0 cc. 



2. Whole egg 150.0 cc. 



3. NasCOa 3.6 to 4.05 g. 



4. Peptone 7.0 g. 



5. NaCl 3.5 g. 



6. Agar 21.0 g. 



Preparation : 



(1) Mix 150.0 cc. water and whole eggs in 

 equal parts, add 3 and shake thor- 

 oughly. (May be filtered thru thin 

 layer of cotton to remove any thick 

 part of egg.) 



(2) Steam (1) for 20 minutes. 



(3) Dissolve 4, 5 and 6 in 700.0 cc. of 

 water. 



(4) Adjustment of reaction of (3) not 

 specified. 



(5) Mix (2) and (3) while (3) is boiling 

 hot. 



(6) Pour medium thick plates and allow 

 to stand open for 20 to 30 minutes. 



Sterilization: Method not given. 



Use: Isolation of cholera vibrio. Author 



reported that colonies by transmitted 



light appeared to be deep in agar, with 



hazy appearance. 

 References: Krumwiede, Pratt and Grund 



(1912 p. 137), Stitt (1923 p. 50). 



1630. Weiss' Nahrstoff Heyden Gelatin Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Gelatin 75.0 g. 



3. Nahrstoff Heyden 4.0 g. 



4. Agar 7.5 g. 



Preparation : 



(1) Dissolve 15.0% (75.0 g.) gelatin and 

 0.8% (4.0 g.) Nahrstoff Heyden in 

 500.0 cc. water. 



(2) Dissolve 1.5% (7.5 g.) agar in 500.0 

 cc. water. 



(3) Mix (1) and (2). 

 Sterilization: Not specified. 

 Use: Bacterial count of water. 

 Reference: Weiss (1920 p. 25). 



1631, Deycke's Alkaline Albumin Gelatin 

 Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Gelatin 50.0 g. 



3. Agar 20.0 g. 



4. .\lkaline albumin 25.0 g. 



5. NaCl 10.0 g. 



6. Peptone 10.0 g. 



