472 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Cultivation and preservation of stock 



cultures of fusiform bacilli. 

 Variants: The authors substituted ascitic 



fluid for horse serum. 

 Reference: Krumwiede and Pratt (1913 



p. 200). 



1633. Dunschmann's Bile Salt Peptone Agar 

 (Bezanfon) 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 30.0 to 40.0 g. 



3. Gelatin 5.0 g. 



4. Sodium taurocholate. 15.0 to 25.0 g. 



5. Lactose 40.0 g. 



6. Peptone (vegetable) . 10.0 g. 

 Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1 (method not given). 

 Sterilization: Method not given. 

 Use: Enrichment of colon-typhoid group. 

 Reference: Bezangon (1920 p. 341). 



1634. Capaldi's Peptone Gelatin Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Peptone (Witte's) 20.0 g. 



3. Gelatin 10.0 g. 



4. Glucose 10.0 g. 



5. NaCl 5.0 g. 



6. KCl 5.0 g. 



7. Agar (2.0%) 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Make alkaline by the addition of 

 10.0 cc. N/1 NaOH. 



(3) Filter. 



Sterilization: Method not given. 



Use: Differentiation between colon and 

 typhoid bacilli. The author reported 

 that coli colonies were large milky colored 

 by reflected and brown by transmitted 

 light. Typhoid colonies were small, 

 glistening and colorless. Abt used a 

 similar medium to produce anthrax 

 spores. 



Variants : 



(a) Abt used 2.0 g. Chapoteaut's peptone, 

 2.0 g. glucose, 20.0 g. gelatin and 

 15.0 g. agar instead of the amounts 

 specified by Capaldi and omitted the 

 KCl. 



(b) Tanner used either 10.0 g. glucose or 

 mannitol. 



(c) Weiss made bacterial counts of water 

 in a medium prepared as follows: 



(1) Dissolve 15.0% gelatin and 1.0% 

 peptone in 500.0 cc. of water. 



(2) Add 0.15% NasCOa to (1). 



(3) Dissolve 1.5% agar in 500.0 cc. of 

 distilled water. 



(4) Mix 500.0 cc. (2) with 500.0 cc. (3). 



(5) Sterilization not specified. 

 References: Capaldi (1896 p. 475), Abt 



(1914 p. 151), Tanner (1919 p. 64), Weiss 

 (1920 p. 25), Heinemann (1922 p. 35). 



1635. Smyth's Egg Trypsinized Peptone 

 Agar 



Constituents: 



1. Ringer's solution (see 

 medium 180) 740.0 cc. 



2. Egg albumin 250.0 cc. 



3 Peptone 10.0 g. 



4. Agar 7.5 g. 



Preparation : 



(1) Sterilize fresh eggs in bichloride of 

 mercury solution followed by alco- 

 hol to remove the bichloride. 



(2) Cut a small window in the side of 

 an egg with a small sharp pointed 

 scissors, in the same manner as one 

 opens an egg to obtain the embryo. 



(3) Remove the white with a sterile glass 

 pipette (3.0 to 5.0 cc.) to a sterile 

 Erlenmeyer flask, without breaking 

 the yolk. 



(4) Dissolve 10.0 g. of Witte's peptone 

 in 200.0 cc. of Ringer's solution 

 (See medium 180) at 80 to 90°C. 



(5) Cool to 45°C. and add 0.5 cc. of tryp- 

 sin powder suspended by shaking in 

 15.0 cc. of distilled water or Ringer's 

 solution and digest for 3 hours at 

 40 to 45 °C. 



(6) Dilute (5) to one liter with Ringer's 

 solution (see medium 180) and boil 

 for 20 minutes and replace loss with 

 distilled water. 



(7) Filter and sterilize (The peptone 

 solution so prepared may be kept 

 for some time in a sterile flask). 



(8) To (3) add an equal quantity of (7). 



(9) Dissolve 15.0 g. agar in one liter 

 Ringer's solution in the Arnold 

 steam sterilizer or autoclave 



(10) Clarify with egg. 



