CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



473 



(11) Distribute in small flasks. 



(12) When ready for use, melt sterile (11) 

 at 100°C., cool to 45°C. in a water 

 bath and then add an equal volume 

 of (8) (albumen peptone solution). 



(13) Keep at 45°C. to prevent solidifica- 

 tion before use. 



Sterilization: Method of sterilization not 



given. 

 Use: To cultivate chick tissue in vitro. 

 Reference: Smyth (1914-15 p. 255). 



1636. Czaplewski's Blood Nahrstoff Heyden 



Agar (Klimmer) 

 Constituents: 



1. Agar 1000.0 cc. 



2. Nahrstoff Heyden (1.0%) . . . 10.0 g. 



3. Blood, pigeon or dog. 

 Preparation : 



(1) Add 1.0% Nahrstoff Heyden to 

 neutral agar. 



(2) Add several drops of pigeon or dog 

 blood to (1). 



(3) Solidify in a thin layer. 

 Sterilization: Not specified. 



Use: Cultivation of influenza bacilli. 

 Reference: Klimmer (1923 p. 227). 



1637. Norris et al. Blood Peptone Agar 

 Constituents: 



1. Water 1000.0 cc. 



2. Peptone 20.0 g. 



3. NaCl (5.0%) 50.0 g. 



4. Glucose 20.0 g. 



5. Agar 20.0 g. 



6. Citrated blood (human). . . . 100.0 cc. 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Adjust slightly alkaline to phenol- 

 phthalein. 



(3) Distribute in 20.0 cc. lots. 



(4) Add 2.0 cc. human citrated blood per 

 20.0 cc. lot of medium. 



Sterilization: Method not given. 

 Use: Cultivation of spirochetes. 

 Reference: Norris, Pappenheimer and 

 Flournoy (1906 p. 281). 



1638. Nicolle's Blood Peptone Agar 

 (Behrens) 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar 20.0 g. 



3. Peptone 20.0 g. 



4. NaCl 5.0 g. 



5. Defibrinated blood, rabbit. . 2000.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Distribute in test tubes in 1.0 cc. lots. 



(3) Shortly before use the desired number 

 of sterile agar tubes are melted in the 

 water bath, cooled to 60°C. and two 

 volumes of defibrinated rabbit's blood 

 are added. 



(4) Mi.x well and solidify in slanting 

 position. 



Sterilization: Sterilize by heating in the 

 autoclave at 105° to 108°C. for 15 minutes. 



Use: Cultivation of Trypanosoma Brucei. 



Variants: Harvey prepared a similar me- 

 dium by dissolving 0.5% peptone, 1.0% 

 NaCl and 1.5% agar in water and mixing 

 one part of this sterile melted agar cooled 

 to 45°C. with one part defibrinated rab- 

 bit's blood also at 45°C. 



References: Behrens (1914 p. 28), Harvey 

 (1921-22 p. 74). 



1639. Kelser's Blood Peptone Agar 



Same as medium 724 but contains no glu- 

 cose and contains 1.5% agar. 



1640. Harvey's Blood Peptone Agar 



Same as variant (b) 724, but solidified by 

 the addition of 3.0% agar. 



1641. Fildes' Blood Peptone Agar 



Constituents: 



1. Water. 



2. Agar. 



3. NaCl. 



4. Peptone (Morson's). 



5. Laked blood. 



6. Serum. 

 Preparation : 



(1) Collect ox or horse blood from a 

 slaughter house. 



(2) Defibrinate the blood by stirring 

 with a sterilized large wooden stick 

 wrapped in gauze. 



(3) Lake the blood by the addition of an 

 equal volume of distilled water. 



(4) Distribute in 200.0 cc. lots into per- 

 fectly fitting glass stoppered bottles. 

 (The author mentions that the me- 

 dium should contain no suspended 

 material, or it will not sterilize with 

 the method employed. However, he 

 does not specify any sedimentation 

 of the mixture after the addition of 

 the water.) 



