476 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



References: Tochtermann (1895 p. 965), 

 Kolle and Wassermann (1912 p. 406), Bes- 

 son (1920 p. 53), Harvey (1921-22 p. 79), 

 Klimmer (1923 p. 221). 



1646. Browning's Telluric Acid Serum Agar 



(Wood) 



Constituents : 



1. Peptone water agar 1000.0 cc. 



2. Serum, sheep 50.0 cc. 



3. Telluric acid (1.0%) 9.0 cc. 



Preparation: (1) Add 50.0 cc. of sterile 



eheep serum, and 9.0 cc. of a 1.0% solution 

 of telluric acid in distilled water to 

 1000.0 cc. of peptone water agar. 



Sterilization : Sterilize the serum by heating 

 at 57°C. on several occasions. Method of 

 sterilizing peptone water agar or telluric 

 acid solution not given. 



Use: Isolation of diphtheria bacilli. 



Reference: Wood (1921 p. 562). 



1647. Teague and Deibert's Serum Peptone 



Agar 



Constituents : 



1. Distilled water. 



2. Peptone. 



3. Agar. 



4. NaCl. 



5. Serum. 



6. Red Blood Cells. 

 Preparation : 



(1) Obtain sheep or rabbit serum from 

 freshly drawn defibrinated blood. 



(2) Heat (1) for 15 minutes at 55°C. 



(3) Add 2.0 cc. of red blood cells (obtained 

 by centrifuging defibrinated rabbit 

 blood and removing the serum) to 

 10.0 cc. of physiological salt solution. 



(4) Keep the temperature of (3) at 100°C. 

 for 3 minutes. 



(5) Shake (4) and allow to cool. 



(6) Centrifuge (5) and obtain the super- 

 natant fluid. 



(7) Prepare a 5.0% peptone solution. 



(8) Mix various amounts of (2), the super- 

 natant fluid from (6) and (7) and add 

 an agar solution containing 5.0 g. 

 NaCl and 20.0 g. agar per 1000.0 cc. 



Sterilization: Not specified. 



Use: To study growth requirements of 

 Unna-Ducrey bacillus. Author reported 

 that excellent growth was obtained if red 



blood cell extract was added. Otherwise 

 no growth. 

 Reference: Teague and Deibert (1922 



p. 70). 



1648. Emlle-Weil's Pleuritic Serum 

 Peptone Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone (Chapoteaut) 20.0 g. 



3. Glucose 8.0 g. 



4. Glycerol 20.0 g. 



5. Agar 24.0 g. 



6. Pleuritic serum (human) . . . 250.0 cc. 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Tube. 



(3) To luke warm fluid (2) add one part 

 human pleuritic serum to four parts 

 agar. 



Sterilization: Not specified. 

 Use: Cultivation of Bacillus leprae. 

 Variants: Emile- Weil gave the following 

 variants: 



(a) Used 40.0 g. glycerol, 4.0 g. glucose, 

 20.0 g. peptone, 20.0 g. agar, and 

 mixed one part human pleuritic serum 

 with two parts agar. 



(b) Dissolved 10.0 g. NaCI, 8.0 g. glucose, 

 16.0 g. peptone and 16.0 g. agar in 

 1000.0 cc. water and mixed 2 parts of 

 this solution with one part human 

 pleuritjc serum. 



Reference: Emile-Weil (1905 p. 798). 



1649. Bacto MacConkey's Agar 

 (Dehydrated) 



Constituents: 



1. Water 



2. Bile, Ox, Bacto 5.0 g. 



3. Lactose, Bacto 10.0 g. 



4. Agar, Bacto 5.0 g. 



5. Peptone, Bacto 20.0 g. 



6. Neutral red 0.05 g. 



Preparation : 



(1) Dissolve 50.0 g. of Bacto MacConkey's 

 Agar (Dehydrated) in 1000.0 cc. dis- 

 tilled water by boiling or autoclaving, 

 preferably the latter. 



(2) Restore the loss by the addition of 

 water if necessary. 



(3) If sterilized at 15 pounds for 20 min- 

 utes pH = 7.0±. 



