CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



477 



Sterilization: Sterilize in the autoclave at 



15 pounds pressure for 20 minutes. 

 Use: General culture medium, and in water 



analysis. 

 Reference: Digestive Ferments Co. (1925 



p. 12). 



1650. Jordan's Basal Peptone Bile Agar 



Constituents : 



1. Bile (ox) 1000.0 cc. 



2. Peptone (Witte) , 10.0 g. 



3. Agar 15.0 g. 



Preparation: 



(1) Dissolve 2, 3 and one of the added 

 nutrients in 1000.0 cc. of undiluted 

 fresh neutral ox bile. Avoid exces- 

 sive heating. 



(2) Filter without adjusting the reaction. 



(3) Tube. 



Sterilization: Sterilize in the autoclave for 

 3 minutes at 15 pounds pressure. 



Use: To show inhibitory action of bile on 

 B. coli. Author reported that both 

 freshly isolated and strains of B. coli 

 long under cultivation were inhibited. 



Added nutrients: Jordan added 10.0 g. 

 lactose. 



Variants : 



(a) Ecker specified the use of Difco pep- 

 tone, used 10.0 g. lactose as an added 

 nutrient and used the medium to 

 study effect of bile on growth of 

 B. typhosus. He reported that some 

 strains were inhibited to a greater 

 degree than others. Approximately 

 the same results were obtained using 

 50.0% or 10.0% bile (diluted with dis- 

 tilled water). 



(b) Ecker used a medium containing 

 10.0 g. glycerol instead of lactose as 

 an added nutrient. The same re- 

 sults were reported as under variant 

 (a) above. 



(c) Ecker used the basal medium with- 

 out any added nutrients. The same 

 results were reported as under 

 variant (a) above. 



(d) Obst used the following medium to 

 determine total count of B. coli in 

 water analysis. 



(1) Sterilize freshly collected ox bile 

 and store at 1°C. until ready for 

 use. Never use bile after one week 

 following collection. 



(2) Dissolve 10.0 g. lactose and 10.0 g. 

 peptone in 1000.0 cc. of (1). 



(3) Dissolve 15.0 g. of agar in (2). 



(4) Filter. Great care must be taken 

 in filtering so that medium be free 

 from precipitate, or colonies can- 

 not be counted. 



(5) Sterilize (method not given). 

 References: Jordan (1912 p. 327), Ecker 



(1918 p. 97), Obst (1919 p. 76). 



1651. Padlewsky's Bile Peptone Agar 

 (Bezangon) 



Constituents: 



1. Water 1000.0 cc. 



2. Peptone 20.0 g. 



3. Bile 35.0 cc. 



4. Lactose 10.0 g. 



5. Malachite green 



(1.0%) 5.0 cc. 



6. Agar 30.0 g. 



7. NasSOj (10.0% 



soln.) 7.5 to 10.0 cc. 



Preparation: 



(1) Dissolve 20.0 g. peptone, 30.0 g. agar 

 and 30.0 cc. beef bile in a liter of 

 water (method not given). 



(2) To 100.0 cc. of one add 0.5 cc. of a 

 1.0% aqueous solution of malachite 

 green and 0.5 cc. of bile. 



(3) Add 0.75 cc. to 1.0 cc. of a 10.0% 

 Na^SOa to each 100.0 cc. of medium. 



Sterilization: Not specified. 



Use: Cultivation of colon-typhoid group. 



Reference: Bezangon (1920 p. 344). 



1652. Northrup's Peptone Bile Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. Peptone 20.0 g. 



4. NaCl. 5.0 g. 



5. Lactose 20.0 g. 



6. Litmus (Standard solution 

 Merck) 10.0 cc. 



7. Bile (ox or sheep) 60.0 cc. 



Preparation : 



(1) Soak 15.0 g. of agar in 500.0 cc. of 

 water over night. 



(2) Dissolve 20.0 g. of peptone and 5.0 g. 

 NaCl in 100.0 cc. hot water. 



(3) After the agar has been digested add 

 (2) to the sugar. 



