478 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(4) Dissolve 20.0 g. lactose in 400.0 cc. 

 hot water. 



(5) Add (4) to (3). 



(6) Add the contents of two ox sheep bile 

 (about 60.0 cc). The bile having 

 been removed under aseptic condi- 

 tions. 



(7) Add 1.0% of a standard solution of 

 Merck's purified litmus (Brown, 

 C. W., Litmus media, 47th Ann. Rep., 

 Michigan Board of Agriculture, pp. 

 127-129). 



(8) Filter. 

 Sterilization: Not specified. 

 Use: Isolation of typhoid bacillus. 

 Variants: (a) Rector reported that colon 



colonies on the following medium were 

 surrounded by a purplish red zone. 



(1) Add 100.0 g, of dried ox bile and 

 10.0 g. Witte's peptone to 1000.0 cc. 

 distilled water and boil. 



(2) Filter. 



(3) Add 1.5% shredded agar to (2) and 

 heat for an hour in the Arnold 

 sterilizer. 



(4) Dissolve 10.0 g. glucose in a small 

 quantity of hot water. 



(5) Filter (3) thru absorbent cotton 

 and cheese cloth. 



(6) Prepare a 1.0% solution of neutral 

 red. 



(7) Add (4) and (6) to the filtrate of (5). 



(8) Mix well. 



(9) Tube in 6 to 10.0 cc. quantities. 

 (10) Autoclave for 10 minutes at 



12 pounds pressure. 

 References: Northrup (1912 p. 420), Rector 

 (1913p. 154),Stitt(1923p. 47). 



1653. Bacto Whey Agar (Dehydrated) 



Constituents : 



1. Distilled water 1000,0 cc. 



2. Whey (dry) 13,0 g. 



3. Peptone (Bacto) 10.0 g. 



4. NaCl 5.0 g. 



5. Agar, Bacto 12.0 g. 



Preparation: 



(1) Dissolve 40.0 g. Bacto Whey Agar 

 (Dehydrated) in 1000.0 cc. of dis- 

 tilled water by boiling or preferably 

 autoclaving. 



(2) If sterilized at 15 pounds pressure 

 for 20 minutes pH = 6.5±. 



Sterilization: Sterilize at 15 pounds pres- 

 sure for 20 minutes. 



Use: Culture medium, particularly for bac- 

 teria in dairy products. 



Reference: Digestive Ferments Co. (1925 

 p. 14). 



1654. Sabouraud and Noire's Whey Peptone 

 Agar (Weil and Noire) 



Constituents : 



1. Water 500.0 cc. 



2. Milk., 1000.0 cc. 



3. Peptone... 10.0 g. 



4. Sucrose 5.0 g. 



5. Urea 1.75 to 2.0 g. 



6. Agar 24.0 g. 



Preparation : 



(1) Precipitate the casein from one liter 

 milk by the addition of 2.0 cc. of HCl. 



(2) Filter thru a wet cloth. 



(3) Dissolve 10.0 g. peptone, 5.0 g. 

 sucrose, 1.75 to 2.0 g. urea in 500.0 cc. 

 water. 



(4) Add one-half volume of (3) to one 

 volume of the filtrate from (2). 



(5) Dissolve 1.6% agar in (4). 



(6) Sterilize (method not given). 



(7) Filter. 



(8) Tube. 



Sterilization: Sterilize again (method not 



given). 

 Use: Cultivation of gonococcus. 

 Variants : Bezangon prepared the medium 



as follows: 



(1) Boil 1000.0 cc. of milk for 5 minutes. 



(2) Add 2.0 cc. of HCl. 



(3) Filter thru linen. 



(4) Add one-half the volume of water to 

 the whey. 



(5) Neutralize by the addition of a 

 10.0% soda solution. 



(6) Autoclave (5) for 10 minutes at 

 120°C. 



(7) Filter. 



(8) Dissolve 1.0% peptone, 1.0% sucrose 

 or glucose, 0.3% urea and 1.6% agar 

 in the filtrate by heating in the 

 autoclave. 



(9) Filter thru paper. 



(10) Distribute in tubes. 



(11) Sterilize for 10 minutes at 110°C. 

 References: Weil and Noire (1913 p. 1322), 



Bezangon (1920 p. 119). 



