CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



481 



(3) Soak 3 in (2). 



(4) Boil for 30 minutes. 



(5) Add 7.5 cc. of normal KOH. 



(6) Neutralize to litmus by the addi- 

 tion of soda solution. 



(7) Add 5.0 cc. of a normal soda solution 

 (143.0 g. crystalline soda per liter). 



(8) Prepare a 10.0% nutrose solution. 



(9) Add 100.0 cc. of (6) to (5). 



(10) Flask. 



(11) Sterilize on each of two successive 

 days. 



(12) Decant the clear agar from the 

 sediment. 



(13) Prepare a 2.0% solution of malachite 

 green 120 (Hochst) in sterile distilled 

 water (do not boil). 



(15) Pour into Petri dishes. 



(16) Leave the plates open until the agar 

 has solidified. 



References: Harvey (1921-22 p. 91), Klim- 

 mer(1923p.212). 



1660. Raskin's Whey Peptone A.gar 



Constituents: 



1. Whey 1000.0 cc. 



2. Glycerol 50.0 cc. 



3. Agar 5.0 to 7.0 g. 



4. Peptone 10.0 g. 



Preparation : 



(1) Add 50.0 cc. of glycerol and 5.0 to 

 7.0 g. finely dried agar to 1000.0 cc. 

 fresh milk. 



(2) Allow to stand for 12 to 14 hours (at 

 room temperature in the winter) and 

 then boil for 75 to 90 minutes over a 

 free flame. In order to prevent loss 

 of water during the boiling place a 

 lid over the container or steam for 

 3 to 3j hours in a steamer. The 

 coagulation of the casein takes place 

 slowly. 



(3) Press thru four folds of linen cloth 

 separating the casein and liquid. 



(4) The reaction of the fluid is slightly 

 acid. 



(5) Pour into a tall glass cylinder and 

 place in a warm thermostat. 



(6) After about 20 or 30 minutes two 

 layers are formed, the lower layer 

 being transparent and fat free, the 

 upper layer yellowish white, con- 

 taining the fat. 



(7) Allow to cool, and remove the fat 

 by means of a spoon. 



(8) Heat the fat free portion to boiling 

 and add 1.0% peptone. 



(9) Add soda to neutralize the reaction . 

 (10) Filter until clear thru a paper in a 

 hot water funnel. 

 Sterilization: Method not given. 

 Use : Cultivation of pathogenic organisms. 

 Variants : 



(a) Raskin added 0.5% XaCl. 

 References: Raskin (1887 p. 358). 



SUBGROUP II-C. SECTION 6 



Basal or complete media containing agar 

 and peptone (or other commercial digests) 

 and extracts or infusions of animal origin. 

 Ai.* Not containing additional organic ma- 

 terials (exclusive of indicators). 

 Bi.* Infusions specified. 

 Ci. Indicators or dyes not added. 

 Di. Meat infusion used. 



Jacobi's Meat Infusion Salt Agar.. 1661 



Zettnow's Meat Infusion Agar 1662 



Besson's Phosphate Infusion Agar. . 1663 



Smith's Infusion Agar 1664 



Francis' Basal Infusion Agar 1665 



Deycke's Alkali Albuminate Agar . . . 1666 



Harvey's Basal Infusion Agar 1667 



Krasnow's et al. Meat Infusion Agar 1668 

 Dj. Infusions of other tissues or special 

 organs, etc. used. 

 Dopter's Liver Infusion Agar 



(White) 1669 



Richardson's Mucose Infusion Agar. 1670 

 Lichtenstein's Blood Clot Infusion 



Agar 1671 



Stuart's Stomach-Liver Infusion 



Agar 1672 



Bailey's Hormone Agar 1673 



Fasiani & Zironi's Veal Autolysate 



Peptone Agar 1674 



Cj. Indicators or dyes added. 

 Harvey's Basal Indicator Infusion 



Agar 1675 



Jordan and Victorson's Lead Ace- 

 tate Infusion Agar 1676 



Elser and Huntoon's Basal Litmus 



Infusion Agar 1677 



Emile-Weil's Neutral Red Infusion 



Agar 1678 



Lentz and Tietz's Malachite Green 



Infusion Agar 1679 



Zielleczky's Phenolphthalein Infu- 

 sion Agar 1680 



Worbitzki's China Green Infusion 

 Agar..,. 1681 



See page 482 for A2 and B2. 



