488 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Joas' Alkaline Serum Agar (Klim- 



mer) 2004 



Muhlen and Hoffman's Glucose 



Serum Agar (Stitt) 2005 



Cantani's Glycerol Serum Agar 



(Besson) 2006 



Kodama's Fuchsin Sulphite Serum 



Agar 2007 



Wassermann's Nutrose Serum Agar. 2008 

 Kliglers' Nasal Secretion Serum 



Agar 2009 



Meyers' Tuberculin Agar 2010 



Douglas' Tellurite Trypsinized Se- 

 rum Agar 2011 



Czaplewski's Alkaline Serum Glu- 

 cose Agar 2012 



Klein's Alkaline Serum Agar 2013 



Frost, Charlton and Little's Milk 



Serum Agar 2014 



Es. Ascitic fluid employed. 



Salomon's Basal Ascitic Fluid Agar. 2015 

 Rosenow's Glucose Ascitic Fluid 



Agar 2016 



Ruediger's Inulin Ascitic Fluid Agar. 2017 

 Klimenko's Glycerol Ascitic Fluid 



Agar 2018 



Veilion's Ascitic Fluid Agar 2019 



Herrold's Phosphate Ascitic Fluid 



Agar 2020 



E4. Bile employed. 



Loeffler's Dye Bile Agar (Abel) .... 2021 

 D3. Containing animal secretions or ex- 

 cretions. 

 El. Milk or its derivatives employed. 



Hasting's Milk Agar 2022 



Valleti's Whey Agar 2023 



Klimmer's Casein Agar 2024 



E2. Milk or its derivatives not employed. 



Mayer's Mucin Agar 2025 



Fichtner's Sputum Agar 2026 



Finger, Ghon and Schlagenhaufer's 



Urine Agar 2027 



D4. Containing materials of plant origin. 

 Eberson's Yeast Infusion Agar 2028 



1661. Jacobi's Meat Infusion Salt Agar 



Constituents : 



1. Meat infusion 1500.0 cc. 



2. Peptone (siccum) 15.0 g. 



3. NaCl 7.5 g. 



4. Agar-agar 22.5 g. 



Preparation : 



(1) Method of preparation of meat in- 

 fusion not given. 



(2) Add 2, 3 and 4 to (1). 



(3) Heat over a free flame until the ma- 

 terials are completely dissolved. 



(4) Make up to the original volume by 

 the addition of water. 



(5) Add Na2C03 or sodium phosphate 

 until the reaction is slightly alkaline. 



(6) Pour into a flask and steam until 

 the albuminous material still to be 



removed is completely separated 

 (usually 2 hours if sodium phosphate 

 is used and longer if Na2C03). 



(7) Filter thru cotton, using compressed 

 air to effect a fast filtration. 



(8) Distribute into smaller flasks. 



(9) Heat in streaming steam for 2 hours 

 (if the glassware has been sterilized 

 in the autoclave). 



(10) Finally distribute into sterile test 

 tubes that have been autoclaved in 

 the autoclave for 2^ hours. (The 

 tubes are contained in an enamel 

 container in the autoclave. The 

 inner temperature reaching about 

 150°C.). 



Sterilization: Not specified. 

 Use: General culture medium. 

 Variants: The following authors prepared 

 similar media as indicated: 

 (a) Schultz (1891). 



(1) Place 500.0 g. of the best quality 

 meat, without fat or tendons, in a 

 glass container fitted with a lid. 



(2) Pour 1300.0 cc. of distilled water 

 over the meat. 



(3) Store in a cool place until the 

 next day. 



(4) Filter thru 4 thicknesses of 

 cloth and press the remaining 

 meat to obtain as much fluid as 



(5) Pour the filtrate into a kettle, 

 add 10.0 g. peptone (siccum), 

 5.0 g, NaCl and the whites of two 

 eggs, beaten up in two or three 

 volumes of water. 



(6) Boil under a gas flame for 15 

 minutes. 



(7) Adjust to faint alkalinity, using 

 phenolphthalein as an indicator, 

 and the end point being a faint 

 red color. 



(8) Pour into an iron kettle, add 

 100.0 cc. distilled water; boil 

 strongly for 5 minutes and filter. 



(9) Add 15.0 g. agar-agar to (8). 



