CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



501 



(5) Suspend the precipitate in distilled 

 water and add concentrated soda 

 solution until part of the precipitate 

 is brought into solution. Dissolve 

 the remainder of the precipitate by 

 prolonged heating in steam. 



(6) Correct the reaction to a weak 

 alkalinity. 



(7) Dry 100.0 cc. of the solution and 

 determine the per cent of dry ma- 

 terial. 



(8) Dilute (6) so that there is 2 to 3.0% 

 solid materials present. 



(9) Dissolve 1.0% peptone, 1.0% NaCl 

 and agar (amount not given) to (8), 

 method not given. 



(10) Neutralize by Dahmen's method 

 with 0.33% soda. 



Sterilization: Not specified. 



Use: Isolation of cholera bacilli from 

 stools. Author reported that this me- 

 dium eliminated practically all other 

 organisms from stools. 



Reference: Deycke (1893 p. 888). 



1667. Harvey's Basal Infusion Agar 



Constituents : 



1. Infusion agar 1000.0 cc. 



Preparation : 



(1) Prepare infusion agar according to 

 variant (v) medium 1661. 



(2) Add one of the added nutrients to (1). 

 Sterilization: Not specified. 



Use : Cultivation of B. acnes and anaerobes. 



Also used as an enrichment medium for 



the colon typhoid group. 

 Added nutrients: The author added one of 



the following: 



(a) Oleic acid 10.0 cc. 



glycerol 20.0 cc. 



(b) Glycerol 20.0 cc. 



oleic acid 1.0 cc. 



(c) Glucose 20.0 g. 



sulphin digotate 10 g. 



(d) Glucose 20.0 g. 



(e) Sodium formate 4.0 g. 



Equal parts agar and 1.0% caffeine 

 solution. 



The combinations (a) and (b) were 

 used to cultivate B. acnes, (c) and (d) 

 used for the cultivation of anaerobes. 

 Agar containing caffeine (e) was used 

 as enrichment medium for members 

 of the colon typhoid group. 

 Reference: Harvey (1921-22 pp. 87, 92, 111). 



1668. Krasnow's et al. Meat Infusion Agar 



Constituents : 



1. Tap water 1000.0 cc. 



2. Veal, Bacto 75.0 g. 



3. Peptone 10.0 g. 



4. NaCl 5.0 g. 



5. Agar 20.0 g. 



Preparation : 



(1) Infuse 75.0 g. of Bacto Veal in 

 500.0 cc. of tap water in the Arnold 

 sterilizer at 100°C. for two hours. 



(2) Allow the coagulum thus formed to 

 settle to the bottom of the container. 

 Allow to cool very slowly. 



(3) Strain thru a wire sieve. 



(4) Dissolve 3 and 4 in (3). 



(5) Adjust to pH 7.9. 



(6) Steam in the Arnold for 15 minutes. 



(7) Filter. 



(8) Add an equal quantity of a 4.0% agar 

 solution. 



Sterilization : Sterilize in the autoclave for 

 45 minutes at 15 pounds pressure. 



Use: Cultivation of streptococci. 



Reference: Krasnow, Rivkin and Rosen- 

 berg (1926 p. 391). 



1669. Dopter's Liver Infusion Agar (White) 

 Constituents: 



1. Water 1000.0 cc. 



2. Liver 500.0 g. 



3. Agar (1.5%) 15.0 g. 



4. Peptone (1.0%) 10.0 g. 



(Witte, Chapoteau or Fair- 

 child) 



5. NaCl (0.5%) 5.0 g. 



Preparation : 



(1) Mix 500.0 g. of well minced calf or 

 beef liver with 1000.0 cc. of distilled 

 water. 



(2) Stir well and infuse over night in the 

 ice box. 



(3) Boil 5 minutes. 



(4) Filter thru paper. 



(5) Make up to original volume. 



(6) Add 1.0% Witte, Chapoteau or Fair- 

 child's peptone, 0.5% NaCl and 1.5% 

 agar. 



(7) Boil 20 minutes and adjust to 4-0.2 

 to phenolphthalein. 



(8) Boil 5 minutes and filter. 

 Sterilization: Sterilize in the Arnold on 



three successive days. 

 Use: General culture medium. Also used 

 to cultivate and carry stock meningococci 



