504 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(7) Boil and add 5.0 g. peptone, 2.5 g. 



NaCl and neutralize again. Boil 

 for 20 minutes. 



(8) Filter. (The filtrate may be divided 

 into two parts, and using half of the 

 infusion as a liquid medium.) 



(9) Add 1.5% agar to (8) and boil until 



solution is complete. 



(10) Neutralize and cool to 68°C. 



(11) Add a well beaten egg to (10) and 

 boil until the egg is thoroly co- 

 agulated. 



(12) Filter and tube. 



Sterilization: Sterilize in the Arnold on 

 each of three successive days for 30 min- 

 utes, and 30 minutes just before inocu- 

 lation. 



Use: Study of bodies found in carcinoma- 

 tous tissue. Author was unable to obtain 

 growth of carcinomatous bodies. 



Reference: Richardson (1900-1 p. 73). 



1671. Lichtenstein's Blood Clot Infusion 

 Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Blood clots 500.0 g. 



3. Peptone 



4. NaCl 



5. Agar 

 Preparation : 



(1) Run the blood clots thru a meat 

 grinding machine. 



(2) Mix (1) with a double amount of 

 distilled water. 



(3) Place in an enamel kettle and boil 

 over a free flame for 10 minutes stir- 

 ring constantly. 



(4) Add concentrated acetic acid 

 (amount not given). 



(5) Boil for five minutes. 



(6) Remove the flame and place the 

 kettle in a slanted position so the 

 precipitated material may settle 

 out. 



(7) Collect the clear liquid after it has 

 cooled. 



(8) Transfer into glass flasks and add 

 the necessary amount (exact amount 

 not given) of peptone, NaCl and 

 agar. 



(9) When the agar has dissolved, neu- 

 tralize and place in the autoclave 

 for 20 minutes. 



(10) Filter. 



Sterilization: Not specified. 



Use: Cultivation of saphrophytic organ- 

 isms. Author reported that diphtheria 

 bacilli and streptococci grew on this me- 

 dium better than on glycerol agar. 

 Saphrophytic organisms requiring sugar 

 in ordinary agar, grew luxuriantly on this 

 medium. 



Reference: Lichtenstein (1915-16 p. 362). 



1672. Stuart's Stomach-Liver Infusion Agar 



Constituents : 



1. Water 1000.0 cc. 



2. HCl 10.0 cc. 



3. Liver 250.0 g. 



4. Stomach, pig 200.0 g. 



5. Peptone, Witte's (1.0%) .... 10.0 g. 



6. Agar (1.2%) 12.0 g. 



Preparation : 



(1) Mix 250.0 g. of finely minced liver 

 and 200.0 g. of that portion of a pig's 

 stomach lining containing the gastric 

 glands, in a liter of water containing 

 10.0 cc. of commercial HCl. 



(2) Incubate the mixture for 18 hours 

 in a water bath at 50°C. 



(3) Make Biuret and tryptophane tests 

 every six hours. 



(4) Stop digestion by heating in the 

 sterilizer in free flowing steam for 

 10 minutes. 



(5) Allow to settle over night in the ice 

 box and decant. 



(6) Add 1.0% Witte's peptone and auto- 

 clave into solution. 



(7) Add 1.2% agar. 



(8) Adjust the reaction to pH = 7.0 

 and boil until the agar is dissolved. 



(9) Adjust the reaction to pH = 7.2 

 to 7.4. 



(10) Tube. 

 Sterilization: Method not given. 

 Use: To study variation in Bacterium 



typhosum. 

 Reference: Stuart (1924 p. 591). 



1673. Bailey's Hormone Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Beef 500.0 g. 



3. Peptone 100 g- 



4. NaCl 5.0 g. 



5. Agar 15.0 g. 



