512 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



meat extract, 10.0 g. Witte's pep- 

 tone and 5.0 g. agar in 1000.0 cc. 

 water. 



(2) Distribute in 10.0 cc. lots into test 

 tubes that will not give up alkali 

 by sterilization. 



(3) Adjustment of reaction or steri- 

 lization not specified. 



(e) Stokes and Hachtel (1909). 



(1) Dry agar at 105° for 30 minutes. 



(2) Dissolve 4.5 g. of (1) in 500.0 cc. 

 distilled water by boiling and make 

 up loss in weight by adding dis- 

 tilled water. 



(3) Dissolve 5.0 g. Liebig's beef ex- 

 tract, 10.0 g. Witte's peptone and 

 8.5 g. NaCl in 500.0 cc. distilled 

 water. 



(4) Mix (3) and (2) and boil 30 

 minutes. 



(5) Make up the loss in weight by the 

 addition of distilled water. 



(6) Filter. 



(7) Adjust the reaction to neutrality 

 (indicator not specified). 



(8) Tube in 9.0 cc. lots. 



(9) Autoclave at 15 pounds pressure 

 for 20 minutes. 



(f) Viehoever (1913) cultivated urea 



splitting organisms on the follow- 

 ing medium: 



(1) Dissolve 6.0 g. Witte's peptone, 

 meat extract (amount not speci- 

 fied) 1.0 g. NaCl and 10.0 g. agar 

 in 500.0 cc. water. 



(2) Add 0.25% NajCOa to (1). 



(3) Tube in 5.0 cc. quantities. 



(4) Sterilize (method not given). 



(g) Bengis (1916). 



(1) Dissolve 30.0 g. powdered agar in 

 1000.0 cc, distilled water. 



(2) Add 10.0 g. Witte's peptone, 5.0 g. 

 NaCl, and 5.0 g. meat extract 

 to (1). 



(3) Filter in incubating flasks. 



(4) Sterilize in Bramhall-Dean auto- 

 clave. 



(h) Meier (1918) made bacterial counts of 

 milk and whey using the following 

 medium : 



(1) Dissolve 15.0 g. agar, 10.0 g. 

 Liebig's meat extract, 10.0 g. 

 Witte's peptone and 5.0 g. NaCl 

 in 1000.0 cc. distilled water. 



(2) Neutralize to litmus and then add 

 10.0 cc. normal soda solution pe? 

 liter of medium. 



(3) Sterilization not specified, 

 (i) Kligler and Defandorfer (1918). 



(1) Dissolve 10.0 g. peptone, 3.0 g. 

 beef extract and 5.0 g. NaCl in 

 1000.0 cc. water. (Meat infusion 

 may be used instead of beef 

 extract.) 



(2) Add 15.0 g. agar to (1) and auto- 

 clave for 1 hour at 15 pounds 

 pressure. 



(3) Cool to 50°C., an egg white is 

 added and steamed in Arnold 

 30 minutes. 



(4) Adjust to pH = 7.4. 



(5) Boil over free flame 6 or 7 minutes, 

 filter, flask and autoclave (length 

 of time not specified). 



(j) Hesse (Ball) (1919). 



(1) Digest 5.0 g. agar in 500.0 cc. of 

 water. 



(2) Dissolve 10.0 g. peptone, 5.0 g. 

 beef extract and 8.5 g. NaCl in 

 500.0 cc. water. 



(3) Mix (1) and (2). 



(4) Filter. 



(5) Adjust the reaction to 1.0%. 



(6) Tube. 



(7) Sterilize in the autoclave, 

 (k) Hesse (Tanner) (1919). 



(1) Dissolve 5.0 g. agar in 500.0 cc, dis- 

 tilled water. 



(2) Dissolve 10.0 g. peptone, 5.0 g. 

 Liebig's beef extract, and 8.5 g. 

 NaCl in 500.0 cc. distilled water. 



(3) Mix (1) and (2). 



(4) Filter. 



(5) Tube. 



(6) Sterilization not specified. 

 (1) Malm (Besson) (1920). 



(1) Dissolve 5.0 g. Liebig's meat ex- 

 tract (or Cibil's meat extract), 

 10.0 g. Chapoteaut's peptone, and 

 5.0 g. NaCl in 1000.0 cc. water. 



(2) Soak 20.0 g. of chopped thread 

 agar in cold water for several 

 hours. Squeeze the water thru 

 a cloth. 



(3) Heat (2) and (1) at 100°C. until the 

 agar is dissolved. 



(4) Readjust the reaction if necessary. 



(5) Allow to cool to 55 or 60°C. 



