CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



517 



2. Agar 15.0 g. 



3. Nahrstoff Heyden 10.0 g. 



4. NaCl 2.5 g. 



5. Meat extract 0-5 g. 



Preparation: 



(1) Dissolve 15.0 g. of agar in 500.0 cc. 

 water. 



(2) Dissolve 3, 4 and 5 in 500.0 cc. water. 



(3) Melt sterile (1) and cool to 44°C. 



(4) Mix equal parts of sterile (3) and 

 sterile (2) at 40°C. 



(5) Pour in sterile plates. 

 Sterilization: Sterilize (1) and (2) sepa- 

 rately (method not given). 



Use: Cultivation of diphtheria bacilli. 

 Variants: The author used 1.0 g. meat 



extract and 5.0 g. NaCl instead of 



amounts indicated. 

 Reference: Glaessner (1900 p. 729). 



1697. Guth's Selenicate Extract Agar 



Same as medium 807, but solidified by the 

 addition of 30.0 g. of agar. 



1698. Conn and Breed's Nitrate Extract 

 Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 150 g. 



3. Peptone 10-0 S- 



4. KNO3 10 g- 



5. Beef extract 3.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Method not given. 



Use : To determine the ability of bacteria to 



reduce nitrates. 

 Variants : 



(a) Committee S. A. B. prepared beef ex- 

 tract agar according to the method of 

 Committee A. P. H. A. (1920 see 

 variant (g) medium 1695 and adjusted 

 the reaction to pH = 6.6 to 7.4. 



(b) Percival sterilized the medium on 3 

 successive days for 20 minutes each 

 day. 



References: Conn and Breed (1919 p. 278), 

 Committee S. A. B. (1920 p. 128), Percival 

 (1920 p. 163), Committee S. A. B. (1923 

 p. 10). 

 1699. Kotlar's Pancreas Peptone Agar 



Constituents : 



1. Water 1000.0 cc, 



2. Powdered pancreas 50.0 g. 



3. Peptone 10.0 g. 



4. NaCl 5.0 g. 



5. Agar 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Neutralize. 

 Sterilization: Method not given. 



Use: Show effect of pancreas extract on 

 growth of organisms. Author reported 

 that the organisms were inhibited by the 

 pancreas extract. 



Reference: Kotlar (1895 p. 153). 



1700. Bacto Andrade Maltose Agar 

 (Dehydrated) 



Constituents : 



1. Water 



2. Beef extract (Bacto) 3,0 g. 



3. Maltose (Bacto) 10.0 g. 



4. Peptone (Bacto) 5.0 g. 



5. Agar (Bacto) 12.0 g. 



6. Andrade Indicator (Difco) . . 0.0275 g. 



7. NaOH 

 Preparation : 



(1) Dissolve 30.0 g. of Bacto Andrade 

 Maltose Agar (Dehydrated) in 

 1000.0 cc. of distilled water by boiling 

 or autoclaving, preferably the latter. 



(2) Tube. 



(3) If sterilized 20 minutes at 15 pounds 

 pH = 7.7±. 



Sterilization: Sterilize in the usual manner, 



avoiding excess heat. 

 Use: General culture medium. 

 Reference: Digestive Ferments Co. (1925 



p. 16). 



1701. Bacto Andrade Mannite Agar 



(Dehydrated) 



Same as 1700, but substituting Bacto 

 Andrade Mannite Agar (Dehydrated) for 

 Bacto Andrade Maltose Agar (Dehydrated) . 



1702. Bacto Andrade Dextrose Agar 



(Dehydrated) 



Same as medium 1700, but using Bacto 

 Andrade Dextrose Agar (Dehydrated) in- 

 stead of Bacto Maltose Agar (Dehydrated) . 



1703. Bacto Andrade Lactose Agar 

 (Dehydrated) 



Same as medium 1700, but using Bacto 

 Andrade Lactose Agar (Dehydrated) in- 



