518 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



stead of Bacto Andrade Maltose Agar 

 (Dehydrated). 



1704. Bacto Andrade Saccharose Agar 

 (Dehydrated) 



Same as medium 1700, but using Bacto 

 Andrade Saccharose Agar (Dehydrated) 

 instead of Bacto Andrade Maltose Agar 

 (Dehydrated). 



1705. Percival's Basal Litmus Extract Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Meat extract, Lemco 5.0 g. 



3. Peptone (Witte's) 10.0 g. 



4. Agar 15.0 g. 



5. Litmus 

 Preparation : 



(1) Soak 15.0 g. agar in 500.0 cc. water 

 for 12 hours. 



(2) Dissolve 2 and 3 in 500.0 cc. water. 



(3) Mix (1) and (2) and boil or steam in 

 the sterilizer for 20 to 30 minutes until 

 solution is complete. 



(4) Neutralize to phenolphthalein and 

 then add 10.0 cc. of normal HCl per 

 1000.0 cc. of medium. 



(5) Cool to 40 to 50°C. and add the white 

 of an egg beaten up in a little water. 



(6) Heat in the steam sterilizer for 

 90 minutes. 



(7) Filter while hot thru a Chardin 

 folded filter paper in a hot water 

 funnel. If not clear, repeat the 

 process. 



(8) Add one of the added nutrients and 

 sufficient litmus solution to give the 

 desired color. (Chalk may be used 

 instead of litmus.) 



Sterilization : Steam for 20 minutes on 3 suc- 

 cessive days. 



Use: General culture medium. 



Added nutrients: The author added 20.0 g. 

 glucose or lactose. 



Reference: Percival (1920 pp. 51, 57). 



1706. Committee S. A. B. Lead Acetate 



Extract Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Beef extract 3.0 g. 



3. Peptone 30.0 g. 



4. Agar 15.0 g. 



5. Lead acetate, basic 



Preparation : 



(1) Prepare extract agar according to 

 Committee A. P. H. A. (1916), see 

 medium 1695. 



(2) Adjust to pH = 7.2 to 7.6. 



(3) Tube in 5.0 cc. quantities. 



(4) Dissolve 1.0 g. of basic lead acetate 

 in 1000 cc. water. 



(5) Add 6.0 cc. of sterile (4) to each tube 

 of sterile (3). 



(6) Incubate to test sterility. 

 Sterilization: Sterilize (3) and (4) sepa- 

 rately (method not given). 



Use: Detection of production of hydrogen 



sulfid. 

 Reference: Committee S. A. B. (1922 



p. 528). 



1707. Conradi's Brilliant Green Picric Acid 

 Extract Agar (Bezanfon) 



Constituents: 



1. Water 900.0 cc. 



2. Agar 30.0 g. 



3. Meat extract, Liebig's 20.0 g. 



4. Peptone, 10.0% soln 100.0 cc. 



5. Brilliant green (1 : 1000) 10.0 cc. 



6. Picric acid (1: 100) 10.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. (Method not 

 given.) 



(2) Make alkaline (indicator not spec- 

 ified. 



(3) To 1.5 liter of agar add 10.0 cc. of 1 

 to 1000 brilliant green and 10.0 cc. of 

 a 1 to 100 picric acid solution. 



Sterilization: Not specified. 



Use: Differentiation of colon-typhoid 

 group. Author reported that typhoid 

 colonies were green and transparent. 

 Paratyphoid colonies were large and the 

 edges turned yellow; coli did not grow. 



Variants : 



(a) Harvey used Lemco extract instead 

 of Liebig's extract. 



(b) Stitt prepared the medium as follows: 



(1) Dissolve 20.0 g. of Liebig's extract 

 and 30.0 g. of agar in 1000.0 cc. of 

 water. 



(2) Adjust the reaction to +0.3 if 

 necessary. 



(3) Filter thru cotton. 



(4) Distribute in 150.0 cc. lots in 

 250.0 cc. Erlenmeyer flasks. 



(5) Sterilize, method not given. 



