CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



525 



1719. Noeggerath's Indicator Agar 

 (Besson) 



Constituents: 



1. Agar 



2. :Methylene blue (sat. soln.) ... 2.0 cc. 



3. Gentian violet (sat. soln.) .... 4,0 cc. 



4. Methyl green (sat. soln.) 1.0 cc. 



5. Chrysoidine (sat. soln.) 4.0 cc. 



6. Fuchsin (sat. soln.) 3.0 cc. 



Preparation : 



(1) Prepare saturated watery solutions 

 of methylene blue, gentian violet, 

 methyl green, chrysoidine and fuchsin. 



(2) Mix 2.0 cc. of methylene blue, 4.0 cc. 

 gentian violet, 1.0 cc. methyl green, 

 4.0 cc. of chrysoidine and 3.0 cc. of 

 fuchsin. 



(3) Add 200.0 cc. of distilled water to (2) 

 and allow to stand several hours. 

 The color should be greenish blue. 

 If not, obtain the original color by 

 adding either blue, green or red dye. 



(4) Add 7 to 10 drops of sterile (3) to a 

 tube of sterile melted nutrient agar. 



Sterilization: Sterilize (3) at 100°C. 

 Use : General culture medium. 

 Reference: Besson (1920 p. 60). 



1720. Gasser's Fuchsin Agar 



Constituents : 



1. Nutrient agar. 



2. Fuchsin. 

 Preparation : 



(1) Add twenty drops of a saturated 

 aqueous solution of fuchsin to a tube 

 of nutrient agar. 



(2) Pour sterile (1) in petri dishes. 

 Sterilization: Method not given. 



Use: Differentiation of colon-typhoid 

 group. Author reported the typhoid cul- 

 ture was red after 24 hours at 37°C. while 

 B. coli communis decolorized the medium. 



Reference: Gasser (1890 p. 463). 



1721. Krumwiede and Pratt's Dahlia Agar 



Constituents: 



1. Agar. 



2. Dahlia. 



Preparation: (1) Add dahlia in the ratio of 

 1 to 100,000 (0.5 cc. of 1.0% dahlia per 

 500.0 cc. agar) to nutrient agar. 



Sterilization: Not specified. 



Use: Detection of cholera vibrio. Author 



reported that cholera vibrio colonies 

 were colored violet. 

 Reference: Krumwiede and Pratt (1913 

 p. 562). 

 1722. Meier's Glucose Infusion Agar 



Constituents : 



1 Water 1000.0 cc. 



2. Beef 500.0 g. 



3. Agar 150 S- 



4. Peptone 10.0 g. 



5. Glucose 5.0 g. 



Preparation : 



(1) Boil 500.0 g. of fat and tendon free 

 beef in 1 liter of water. 



(2) Filter, 



(3) Dissolve 3, 4 and 5 in (2). 



(4) Neutralize by the addition of KOH. 

 Add KOH until turmeric paper is 

 turned quite weakly brownish red. 



Sterilization: Not specified. 



Use: Bacterial count of milk. Goss, Bar- 

 barin and Haines cultivated B. chauvoei 

 on a similarly prepared medium. 



Variants: Goss, Barbarin and Haines pre- 

 pared a similar medium as follows: 



(1) Prepare beef infusion from 500.0 g. 

 beef and 1000.0 cc. water. (Exact 

 method not given.) 



(2) Add 10.0 g. peptone, 5.0 g. NaCl and 

 20.0 g. Dextrose to (1). 



(3) Adjust to pH = 8.2. 



(4) Add 2% agar. 



(5) Heat in flowing steam until agar is 

 dissolved. 



(6) Clarify with egg albumin. 



(7) Filter and tube. 



(8) Autoclave at 15 pounds pressure for 

 20 minutes. 



References: Meier (1818 p. 436), Goss, Bar- 

 barin and Haines (1921 p. 615). 



1723. Kitchens' Glucose Infusion Agar 



Constituents : 



1. Distilled water 2000.0 cc. 



2. Beef, lean 1000.0 g. 



3. Peptone 40.0 g. 



4. KNO3 4.0 g. 



5. Glucose 4.0 g. 



6. Agar 60.0 g. 



Preparation : 



(1) Stir 1000.0 g. of lean beef into 

 1000.0 g. of water. 



(2) Incubate at 37°C. for 48 hours. 



