528 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



B. typhi browned the medium along the 



line of growth. 

 B. paratyphi B produced browning and 



gas. 

 B. paratyphi A gave gas but no browning. 

 B. dysenteriae gave neither gas nor 



browning. 

 Variants: Harvey added 4 drops of sterile 

 30% glucose solution at 45°C. and 2 drops 

 of a sterile 5.0% lead subacetate at 45°C. 

 to 10.0 cc. of melted sterile infusion agar 

 (see variant (v) 1661). 

 Reference: Kligler (1917 p. 1043), Harvey 

 (1921-22 p. 107). 



1727. Gage and Phelps' Neutral Red 

 Infusion Agar 

 Constituents : 



1. Meat infusion 1000.0 cc. 



2. Agar 10.0 g. 



3. Peptone (Witte) 10.0 g. 



4. Glucose 3.0 g. 



5. Neutral red (1.0% watery 

 solution) 10.0 cc. 



Preparation : 



(1) Prepare 1000.0 cc. of meat infusion. 



(2) Dissolve 2, 3 and 4 in (1). 



(3) Add 10.0 cc. of a 1.0% watery solution 

 of neutral red to (2). 



Sterilization: Not specified. 



Use: To study the neutral red reaction by 

 members of the colon group. Color 

 changes from red to yellow if the reaction 

 is positive. 



Reference: Gage and Phelps (1902 p. 408). 



1728. Harvey's Malachite Green Infusion 

 Agar 

 Add 10.0 g. glucose and from 16 to 25.0 cc. 

 of a 1-1000 malachite green solution to 

 1000.0 cc. infusion agar, (see variant v 

 medium 1661) with a reaction of 0.3% acid 

 to phenolphthalein. 



1729. Endo's Fuchsin Sulphite Infusion 

 Agar 

 Constituents : 



1. Water 1000.0 cc. 



2. Beef 500.0 g. 



3. Peptone 10.0 g, 



4. NaCl 5.0 g. 



5. Agar 30.0 g. 



6. Lactose (C. P.) 10.0 g. 



7. Fuchsin (alcoholic solution) 5.0 cc. 



8. Sodium sulfite (10.0% soln.). 25.0 cc. 



Preparation : 



(1) Add 2, 3, 4 and 5 to 1. 



(2) Boil well (time not given). 



(3) Filter and neutralize. 



(4) Add 10.0 cc. of 10 0% soda solution to 

 make alkaline. 



(5) Add 10.0 g. of chemically pure lac- 

 tose and 5.0 cc. of a filtered alcoholic 

 fuchsin solution (strength not given). 



(6) Add 10,0 cc. of a 10.0% sodium sulfite 

 solution. This nearly decolorizes the 

 red medium. The sulfite solution 

 must be kept in a well stoppered 

 bottle or prepared fresh. 



(7) Distribute in 15.0 cc. lots, 



(8) Store sterile (7) in a dark place until 

 ready for use. 



Sterilization: Sterilize for 30 minutes in a 

 steaming apparatus. 



Use: Diagnosis of typhoid fevers. Author 

 reported that typhoid colonies were 

 colorless; coli colonies red, and after 

 24 hours a green shiny fuchsin crystal- 

 like upper surface appeared on them. 



Variants: The following authors have pre- 

 pared the medium as indicated below: 



(a) Reitz. 



(1) Boil 500.0 g. of finely chopped beef 

 with 1 liter water, 10.0 g. Witte's 

 siccum peptone and 30.0 g. agar. 



(2) Filter and neutralize. 



(3) Make alkaline by adding 10.0 cc. 

 of a 10.0% soda solution. 



(4) Add 10.0 g. of c. p. lactose and 

 5.0 cc. of a filtered alcoholic fuch- 

 sin solution. 



(5) Decolorize the red medium by the 

 addition of 25.0 cc. of a freshly 

 prepared 10,0% Na2S03 solution. 



(6) Sterilization not specified. 



(b) Stroszner prepared a regenerated 

 Endo agar as follows: 



(1) Place the Endo agar in enamel 

 containers and melt in streaming 

 steam. 



(2) Measure the melted agar in a 

 graduated cylinder. 



(3) To a liter of (2) add 50.0 g. of 

 powdered charcoal and boil for 

 30 to 40 minutes in a steamer 

 (Stir). 



(4) Cool to 50°C. and add 50.0 cc. 

 defibrinated blood per liter of 

 agar at room temperature. Add 



