532 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Preparation of horse or beef infusion 

 not given. 



(2) Dissolve 10.0 g. peptone, 5.0 g. NaCl 

 and 30.0 g. agar in 1. 



(3) Clarify. 



(4) Neutralize to litmus. 



(5) Add 50.0 to 70.0 cc. of tenth normal 

 NaOH and 10.0 g. lactose dissolved in 

 20.0 or 30.0 cc. water. 



(6) Dissolve 0.6 g. NaOH and 0.8 g. ali- 

 zarine in 100.0 cc. distilled water and 

 boil several minutes. 



(7) Add (6) to (5). 



(8) Thoroly mix sterile (7) and pour into 

 sterile plates. 



Sterilization: Sterilize by steaming for 

 30 minutes. 



Use : Detection of typhoid and paratyphoid 

 bacteria. Author reported that B. coli 

 lightened the medium coloring it yellow, 

 typhoid bacilli, paratyphoid and enteri- 

 tidis bacilli left the medium opaque and 

 formed greyish blue colonies. The addi- 

 tion of malachite green completely in- 

 hibited the colon bacteria; however, 40 

 to 48 hours were required for the develop- 

 ment of the typhoid colonies. 



Variants: Klimmer added 1.7 cc. of a 0.1% 

 malachite green solution to each 100.0 cc. 

 of medium. 



Reference: Klimmer (1923 p. 215). 



1733. Harvey's Starch Agar 



Constituents: 



1. Infusion agar 1000.0 cc. 



2. Starch (water soluble) 2.0 g. 



Preparation : 



(1) See medium 1661, Variant (v) for the 

 preparation of infusion agar. 



(2) Add 2.0 g. water soluble starch to 

 1000.0 cc. of (1). 



Sterilization: Not specified. 

 Use: General culture medium. 

 Reference: Harvey (1921-22 p. 112). 



1734. Gassner's Metachrome Yellow Water 

 Blue Infusion Agar 



Constituents: 



1. Infusion agar 2000.0 cc. 



2. Metachrome Yellow (II RD) 



(2.0%) 125.0 cc. 



3. Water blue (6B extra P) 



(1.0%) 175.0 cc. 



4. Lactose 100.0 g. 



Preparation : 



(1) Prepare meat infusion agar (yeast 

 may be used instead of meat). 



(2) Neutralize to litmus. 



(3) Prepare a 2.0% solution of meta- 

 chrome yellow (II RD) in water. 



(4) Boil two minutes. 



(5) Prepare a 1.0% water blue (6B extra 

 P) in solution in water and dissolve 

 100.0 g. lactose in 175.0 cc. of the 

 solution. 



(6) Boil (5) 10 minutes. 



(7) Add 125.0 cc. of (4) and 175.0 cc. of 

 (6) to 2000.0 cc. (2). 



Sterilization: Method not given. 



Use: Detection of dysentery or typhoid 

 bacilli. The author reported that Bad. 

 coli gave a deep blue medium. Typhoid 

 and dysentery gave a yellowish medium. 

 Medium was green. This medium inhib- 

 ited neither the typhoid, dysentery nor 

 coli organisms. 



Reference: Gassner (1917-18 p. 221). 



1735. Torrey's Brom Cresol Purple Lactose 

 Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Beef heart 500.0 g. 



3. Peptone 10.0 g. 



4. Agar 15.0 g. 



5. Lactose 1.0 g. 



6. Brom Cresol Purple (Sat. 



ale. soln.) 0.75 cc. 



Preparation : 



(1) Boil 500.0 g. finely chopped beef 

 heart (other meat material may be 

 used) in one liter of water for 15 

 minutes over a free flame 



(2) Strain through canton flannel and 

 absorbent cotton. 



(3) Make up the loss in filtrate and add 

 10.0 g. peptone and 15.0 g. flaked 

 agar. 



(4) Heat in the Arnold until solution is 

 complete. 



(5) Adjust to pH = 7.4 and place in the 

 Arnold for 30 minutes. 



(6) Readjust the reaction if necessary. 



(7) Filter thru cotton and add 1.0 g. 



