534 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(11) To the clear, straw colored filtrate, 

 whose reaction should be neutral, or 

 at the most slightly alkaline, add 8 

 to 10.0% glycerol with continual 

 shaking. 



(12) Distribute in test tubes or flasks. 

 Sterilization: After 3 appropriate steriliza- 

 tions (exact details not given), the 

 medium may be slanted or remain 

 upright. 



Use: Cultivation of influenza bacilli, tu- 

 bercle bacilli and other pathogenic 

 organisms. 



Reference: Kowalski (1890, p. 245). 



1738. Henssen's Glycerol Kidney Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Kidney 500 g. 



3. Agar 15.0 g. 



4. Peptone 10.0 g. 



5. Glycerol 30.0 g. 



6. NaCl 5.0 g. 



Preparation : 



(1) Prepare kidney infusion in the same 

 manner as beef infusion (exact 

 method not given). 



(2) Dissolve 2, 3, 4, 5 and 6 in (1). 

 Sterilization: Not specified. 



Use: Cultivation of pathogenic organisms. 

 Reference: Henssen (1895 p. 406). 



1739. Harvey's Mannitol Infusion Agar 



Constituents: 



1. Infusion agar 1000.0 cc. 



2. Mannitol 10.0 g. 



Preparation : 



(1) See variant (v) medium 1661 for 

 preparation of infusion agar. 



(2) Add 1.0 g. mannitol to 100.0 cc. of (1). 

 Sterilization: Not specified. 



Use: General culture medium. 

 Reference: Harvey (1921-22 p. 111). 



1740. Omelianski's Formate Agar 



Constituents: 



1. Meat infusion 1000.0 cc. 



2. Agar 20.0 g. 



3. Sodium formate 10.0 g. 



4. Phenolphthalein solution . . . 200.0 cc. 

 Preparation : 



(1) Prepare a phenolphthalein solution 

 by dissolving 0.5 g. phenolphthalein 

 in 100.0 cc. of a mixture of equal parts 



of water and alcohol. Dilute with 20 

 parts water. 



(2) Composition of usual infusion broth 

 not given. (A 0.5% meat extract 

 may be substituted for meat in- 

 fusion.) 



(3) Dissolve 2 and 3 in (2). 



(4) Add200.0cc.of (l)to(3). 

 Sterilization: Method not given. 



Use: To study decomposition of formic 

 acid. Author reported that after 24 

 hours the agar was colored red near the 

 colonies. After several days the whole 

 plate had turned red. 



Reference: Omelianski (1903-04 p. 186). 



1741. Harvey's Salicylate Infusion Agar 



Constituents : 



1. Infusion agar 1000.0 cc. 



2. Sodium salicylate (1.0<%) • • • 10-0 g- 

 Preparation : 



(1) Add 1.0% sodium salicylate to 

 Harvey's Infusion Agar (see variant 

 (v) of medium 1661). 

 Sterilization: Not specified. 

 Use: Cultivation of molds and torulae. 



Bacterial growth is inhibited. 

 Reference: Harvey (1921-22 p. 93). 



1742. de Gasperi and Savini's Glucose 

 Lactose Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Beef 500.0 g. 



3. Agar 12.0 g. 



4. Peptone (Witte) 10.0 g. 



5. NaCl 5.0 g. 



6. Glucose 4.0 g. 



7. Lactose 4.0 g. 



Preparation : 



(1) Mix 500.0 g. finely chopped lean beef 

 with 1000.0 cc. of water, and allow to 

 stand for 24 hours at room tempera- 

 ture. 



(2) Boil for 15 minutes over a free flame. 



(3) Filter thru a double chardin filter 

 paper and press out the residue. 



(4) Add 12.0 g. of finely chopped agar, 

 10.0 g. of Witte's peptone and 5.0 g. 

 of NaCl, and boil over a free flame, 

 or at 110°C. in the autoclave, until 

 the agar is dissolved. 



(5) Cool to about 55°C. and add 4.0 g. of 

 glucose and 4.0 g. lactose and a little 



