CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



537 



1747. Hulton-Frankel's Inositol Infusion 



Agar 



Constituents : 



1. Infusion agar (3.0%) 1000.0 cc, 



2. Dextrin 10.0 g. 



3. Inositol 10.0 g. 



4. Litmus (Griibler's) 

 Preparation : 



(1) Method of preparation or exact 

 composition of 3.0% infusion agar not 

 given. 



(2) Adjust reaction to about +0.7 acid. 



(3) Sterilize in the autoclave (time or 

 pressure not given). 



(4) Readjust reaction to +0.7 acid. 



(5) Add 2 and 3. (Inositol prepared 

 according to Nelson (Jour. Am. 

 Chem. Soc. 37: 1552, 1915) and 

 dextrin, Merck's C. P.) 



(6) Add sterile solution of litmus 

 (Griibler's) till a light violet color is 

 obtained. 



(7) Tube and slant after final steriliza- 

 tion. 



Sterilization: Partial sterilization given in 

 step (3) above. Sterilize (7) once in the 

 Arnold sterilizer as further heating tends 

 to break up the carbohydrates. Incubate 

 24 hours and discard tubes showing 

 growth. 



Use : To differentiate between typhoid and 

 paratyphoid A and B bacilli. Author 

 reported that typhoid bacillus fermented 

 dextrin-inositol with acid formation. 

 Para-typhoid bacillus B fermented dex- 

 trin-inositol with acid and gas formation. 

 Para-typhoid bacillus A did not ferment 

 dextrin-inositol. Shiga-Kruse and Hiss- 

 Russel dysentery types did not ferment 

 dextrin-inositol. Flexner Rosen dysen- 

 tery types fermented dextrin-inositol. 

 B. typhi murium and B. pullorum did not 

 ferment dextrin-inositol. B. aerogenes 

 fermented dextrin-inositol with gas 

 formation. 



Reference: Hulton-Frankel (1918 p. 380). 



1748. Robinson and Rettger's Opsine 



Infusion Agar 

 Constituents : 



1. Beef infusion 1000.0 cc. 



2- Agar 15.0 g. 



3. Opsine (2.0%) 20.0 g. 



4. NaCl 5.0 g. 



5. KH2PO4 5.0 g. 



6. Sodium citrate 2.0 g. 



7. MgS04 2'og' 



8. Glucose 5.0 g. 



9. Glycerol 60.0 g. 



Preparation : 



(1) Prepare 1000.0 cc. of beef infusion. 



(2) Dissolve 2, 3, 4, 5, 6, 7, 8 and 9 in (1). 



(3) Adjust faintly alkaline to litmus. 

 Sterilization: Sterilize at 12 pounds for 15 



minutes. 

 Use: General culture medium. The author 



reported that 2.0% opsine gave better 



general medium than using 1.0%. 



Medium supported the growth of some 



pathogenic forms. 

 Variants: The authors used only 1.0% 



opsine instead of 2.0%. 

 Reference: Robinson and Rettger (1918 p. 



214). 



1749. Lubenau's Lactose Caffeine Agar 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Beef 5OO.O g. 



3. Agar (2.0 to 3.0%) . . . 20.0 to 30.0 g. 



4. Peptone 3.0 g. 



5. NaCl (0.5%) 5.0 g. 



6. Lactose (0.5%) 5.0 g. 



7. Caffeine 



8. Litmus 

 Preparation : 



(1) Boil 500.0 g. of finely chopped lean 

 beef with one liter distilled water for 

 30 minutes. 



(2) Filter, and make up to one liter. 



(3) Add 3, 4, and 5 to (2) and dissolve by 

 boiling in a salt water bath. 



(4) Neutralize to litmus. 



(5) Boil and filter. 



(6) Add 60.0 cc. of sterile litmus solution 

 (preparation not given) to hot ster- 

 ile (5). 



(7) Mix thoroughly, and allow to cool. 



(8) Add 110.0 cc. of a sterile 6.0% caffeine 

 solution to (7). Mix well. 



(9) Pour in plates. 



Sterilization: Sterilize (5), method not 



given. 

 Use : Detection of typhoid bacteria. 

 Variants: Harvey added 5.0 g. lactose, 



110.0 cc. of 6.0% caffeine, 60.0 cc. litmus 



