CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



539 



of nutrients + 2.0% or 0.2% urea + 

 0.1%NaoCO3. 

 (d) Basal medium with 0.3 concentration 

 of nutrients + 0.2% (NH4)2C03 + 

 0.1% NaaCOs. 

 Reference: Viehoever (1913 p. 214). 



1753. Bacto Dextrose Agar (Dehydrated) 



Constituents: 



1. Distilled water 



2. Beef extract (Bacto) 3.0 g. 



3. Peptone (Bacto) 5.0 g. 



4. Glucose (Bacto) 10.0 g. 



5. Agar (Bacto) 15.0 g. 



Preparation : 



(1) Dissolve 33.0 g. of Bacto Dextrose 

 Agar (Dehydrated) in 1000.0 cc. 

 distilled water by Ijoiling or prefer- 

 ably autoclaving for 10 minutes at 15 

 pounds. 



(2) If sterilized 10 minutes at 15 pounds 

 pH = 7.4±. 



Sterilization: Sterilize in the usual manner. 



Use: General culture medium. Add 8.0 g. 

 NaCl per liter to prevent hemolysis when 

 using as a base for blood agar. 



Reference: Digestive Ferments Co., (1925 

 p. 11). 



1754. Henneberg's Glucose Extract Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose 20.0 to 50.0 g. 



3. Meat extract 10.0 g. 



4. Peptone 10.0 g. 



5. NaCl 2.0 g. 



6. Agar 10.0 g. 



Preparation : 



(1) Dissolve 2,3, 4, 5 and 6 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of acetic acid bacteria, 

 B. pasteurianutn, B. oxijdans, B. aceti, 

 B. kutzingianum. Other investigators 

 cultivated a large variety of organisms on 

 similar media. 

 Variants : 



(a) Gottheil cultivated organisms found 



in the soil and on the roots and 



rhizomes of plants: 



(1) Dissolve 2.0 g. NaCl, 8.0 g. meat 



extract, Liebig's, and 12.0 g. 



Witte's peptone in 500.0 cc. of 



water. 



(2) Neutralize with concentrated 

 NaaCOs solution. 



(3) Heat for a time in a sterilizer. 



(4) Filter. 



(5) Soak 16.0 g. of agar in 500.0 cc. 

 water for about three hours. 



(6) Mix (4) and (5). 



(7) Allow to stand for 3 hours at 100°C. 



(8) Neutralize once more with Na2C03 

 and heat for a short time. 



(9) Filter. 



(10) Add 10.0 g. glucose and sterilize 

 on three successive days. 



(b) Bredeman cultivated Bac. amylo- 

 bacter and prepared the medium as 

 follows: 



(1) Wash 215.0 g. of agar in running 

 tap water for three or four hours. 



(2) Add water to (1) until the total 

 weight is 10 kilograms. Dissolve 

 the agar in the water. 



(3) Clarify with egg white and filter. 



(4) Dissolve 7.2 g. Witte's peptone, 

 6.0 g. dextrose, 4.8 g. Liebig's meat 

 extract and 1.2 g. NaCl in 200.0 

 cc. of water. 



(5) Adjust the reaction so that it is 

 slightly alkaline. 



(6) Boil and filter. 



(7) Liquify400.0g. of (3). 



(8) Mix (7) and (6). 



(9) Sterilize on three successive days 

 in streaming steam. (The author 

 also prepared the medium by 

 dissolving the constituents in the 

 water and adjusting the reaction 

 to a slight alkalinity to litmus.) 



(c) Zikes used medium containing 1.0% 

 agar, 1.0% peptone, 1.0% meat 

 extract, 0.5% NaCl and 0.25% glu- 

 cose and cultivated apiculatus yeast, 

 Torula alba, Torula Molischiana. 

 Mycoderma cervisiae. Blastoderma 

 salmonicolor. 



(d) Bachmann cultivated obligate ana- 

 erobes using Gottheil's medium, but 

 used 1.0% peptone instead of 1.2%. 



e) Burckhardt and Enriquez cultivated 

 diphtheria bacilli on a medium pre- 

 pared as follows: 



(1) Dissolve 10.0 g. agar, 10.0 g. 

 peptone, 10.0 g. meat extract, 5.0 

 g. NaCl in 1000.0 cc. of water. 



(2) Neutralize to litmus. 



