550 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



dysentery bacillus, cholera spirillum and 

 other intestinal forms. 

 Reference: Stokes and Hachtel (1913 p. 



1764. Committee S.A.B. Starch Extract 

 Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Water sol. starch 2.0 g. 



3. Beef extract 3.0 g. 



4. Peptone 5.0 g. 



5. Agar 15.0 g. 



Preparation : 



(1) Prepare beef extract agar according 

 to Committee A.P.H.A. (1916-1917). 

 (See extract agar 1694). May be 

 clarified with white of egg. 



(2) Adjust to pH = 6.6 to 7.4. 



(3) Add starch. 

 Sterilization: Method not given. 

 Use: Standard culture medium. 

 References: Committee S. A. B. (1918 p. 



116), (1923 p. 10), Ball (1919 p. 78). 



1765. Coplin and Bevan's Glycerol Extract 



Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Meat extract 5.0 g. 



3. Peptone (albumin) 10.0 g. 



4. Glycerol 62.0 g. 



5. Agar (1.5%) 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Clear and filter in the same way as 

 other media. (Method not given) . 



Sterilization: Not specified. 



Use: Cultivation of Micrococcus pyogenes 

 aureus. Authors reported that 3 or 4 

 days after inoculation the medium 

 became opaque, and after 5 or 6 days the 

 medium appeared as if tapioca was mixed 

 with it. 



Reference: Coplin and Bevan (1892 p. 70). 



1766. Piettre and de Souza's Citric Acid 



Extract Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Meat extract 0.7 g. 



3. Peptone (Chapoteaut) 10.0 g. 



4. NaCl 5.0 g. 



5. Citric acid 5.0 g. 



6. Agar 18 to 20.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 by heating on 

 the salt water bath. 



(2) Neutralize to phenolphthalein using 

 10.0% soda. 



(3) Add 18.0 to 20.0 g. of agar to boiling 

 (2). 



(4) Clarify (Method not given). 



(5) Tube in about 10.0 cc. lots. 



(6) Add by means of a sterile pipette 

 graduated in tenths, 0.5 cc. of a 10.0% 

 sterile citric acid solution to each 

 tube of melted sterile (5) cooled to 

 50°C. 



(7) Mix thoroly and slant. 

 Sterilization: Method not specified. 

 Use: Isolation of molds and fungi, Mucor, 



Rhizopus, Penicillium, Aspergillus and 

 Scelerotinia. Authors reported that it 

 may be necessary to add 8 or even 10.0 g. 

 of citric acid to keep down bacterial 

 growth. 

 Reference: Piettre and de Souza (1922 p. 

 336). 



1767. Fawcus' Dye Bile Salt Agar 



(Bezanfon) 



Constituents : 



1. Water 900.0 cc. 



2. Agar 30.0 g. 



3. Meat extract, Liebig's 20.0 g. 



4. Peptone (10.0% soln.) 100.0 cc. 



5. Brilliant green (1 : 1000) 10.0 cc. 



6. Picric acid (1:100) 10.0 cc. 



7. NaCl 20.0 g. 



8. Sodium taurocholate 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 7 and 8 in 1. 



(2) Make alkaline (Indicator not speci- 

 fied). 



(3) To 1.5 liters of agar add 10.0 cc. of 

 1-1000 brilliant green and 10.0 cc. of a 

 1-100 picric acid solution. 



Sterilization: Method not given. 



Use: Cultivation of colon typhoid group. 



Reference: Bezangon (1920 p. 345). 



1768. Percival's Urea Extract Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Beef extract (Lemco's) 5.0 g. 



3. Peptone (Witte's) 10.0 g. 



4. Agar 15.0 g. 



5. Urea 20.0 g. 



