CULTURE MEDIA FOR CULTIVATIOX OF MICROORGANISMS 



551 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) If acid, neutralize with (NH4)2C03. 

 Sterilization : Sterilize in the usual manner. 



(Method not given). 



Use: Cultivation of organisms using urea. 

 Urobacillus Pasteurii. Author reported 

 that urea fermenting colonies were sur- 

 rounded with small white crystals of 

 calcium carbonate. 



Reference: Percival (1920 p. 224). 



1769. Gaehtegen's Caffeine Endo Agar 



Constituents: 



1. Water 1000 cc. 



2. Meat extract (Liebig's) 20.0 g. 



3. Peptone (Witte siccum) 20.0 g. 



4. NaCl 10.0 g. 



5. Agar 80.0 g. 



6. Lactose 20.0 g. 



7. Fuchsin (10.0% alcoholic 

 solution 10.0 cc. 



8. Sodium sulphite (in 50.0 cc. 



water) 5.0 g. 



9. Caffeine 6.6 g. 



Preparation : 



(1) Add2, 3, 4and5tol. 



(2) Place in the autoclave at 110°C. for 

 two hours. 



(3) When agar is completely dissolved, 

 filter thru cotton. 



(4) Add N/1 NaOH until it is 1.5% 

 alkaline to phenolphthalein. 



(5) Add 20.0 g. lactose, 10.0 cc. of a 10.0% 

 alcoholic fuchsin solution and a 

 sodium sulphite solution (5.0 g. of 

 sodium sulphite in 50.0 cc. water). 



(6) Dissolve 0.33% chemically pure 

 crystalline caffeine in (5). 



(7) Pour into sterile Petri dishes. 

 Sterilization: Not specified. 



Use: Diagnosis of typhoid fever. After 24 

 hours transparent typhoid colonies ap- 

 peared about 1 to 1.5 mm. in diameter. 

 After 30 hours the colonies were 2 to 3 

 mm. in diameter, round, plate form, 

 completely colorless, by transmitted 

 light, a delicate pink in reflected light. 

 B. coli colonies, if any, were red. 

 Caffeine inhibited B. coli. 



Reference: Gaehtegens (1905 p. 636). 



1770. Wilson and Darling's Brilliant Green 



Bile Salt Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Lemco 10.0 g. 



3. Agar 30.0 g. 



4. Peptone 20.0 g. 



5. Sodium taurocholate 5.0 g. 



6. NaCl 5.0 g. 



7. Lactose 5.0 g. 



8. Brilliant green (1.0% 



solution) 4.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Adjust the reaction to a +10 (Eyre) 



(3) Distribute in 100.0 cc. quantities. 



(4) Add 0.4 cc. of a 1.0% solution of 

 brilliant green to each 100.0 cc. of 

 melted medium, cooled to 50°C. when 

 ready to pour in plates. 



Sterilization: Not specified. 



Use: Cultivation of colon typhoid group. 

 Authors reported that the reaction must 

 be +10 (Eyre). If more acid B. coli will 

 grow. If more alkaline B. typhosus is 

 inhibited. Some B. coli may grow. 

 These produce acid and allow for the 

 development of others. 



Reference: Wilson and Darling (1918 p. 

 105). 



1771. Olszewski and Kohler's Endo Bile 



Salt Agar 



Constituents: 



1. Water 2000.0 cc. 



2. Agar 60.0 g. 



3. Meat extract 20.0 g. 



4. Peptone 20.0 g. 



5. NaCl 10.0 g. 



6. Sodium taurocholate 2.0 g. 



7. Lactose 20.0 g. 



8. Fuchsin (alcoholic) 10.0 cc. 



9. Sodium sulphite (10.0%).... 50.0 cc. 

 Preparation : 



(1) Soak 60.0 g. agar over night in 

 1000.0 cc. of water. 



(2) Boil for two to two and a half hours. 



(3) Add 3, 4 and 5 to 1000.0 cc. of water 

 and boil an hour. 



(4) Filter. 



(5) Mix (4) and (2). 



