CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



553 



Dilutions of a bile culture of a typhoid 

 suspect was incubated for 24 hours and a 

 series of dilutions made. These were 

 then plated on the medium and incubated 

 for 24 hours. Typhoid bacillus or para- 

 typhoid bacillus colonies were medium 

 sized, with concentric rings and showed a 

 distinct pink color. The pink color en- 

 abled one to distinguish them from 

 Bacillus typhosus or Bacillus alcaligenes. 

 Colon colonies were small, moist and 

 concentrated. The spirillum of cholera 

 and Nassik spirillum formed a blue 

 colony. Colonies were bluish, concentric 

 with an opaque nucleus-like center and 

 varying diameters from 5 to 15 milli- 

 meters. 



Reference: Stokes and Hachtel (1909 p. 

 41), (1913 p. 346). 



1774. Schniirer's Saponin Glycerol Agar 



Constituents: 



1. Beef extract agar 



(3.0%) 1000.0 cc. 



2. Glycerol 30.0 cc. 



3. Saponin (Merck's) . . . 10.0 to 80.0 g. 

 Preparation : 



(1) Prepare a beef extract agar 3.0% 

 (contains 1.0% peptone and 0.5% 

 NaCl). 



(2) Add 3.0% glycerol and 1.0 to 8.0% 

 saponin to (1). 



Sterilization: Not specified. 



Use: To study acid fastness of tubercle 

 bacilli and timothy bacilli. Author re- 

 ported that acid fastness was not changed 

 by the presence of saponin in medium. 



Reference: Schniirer (1922-23 p. 15). 



1775. Bacto Russell Double Sugar Agar 



(Dehydrated) 



Constituents: 



1. Distilled water 



2. Peptone (Bacto) 10.0 g 



3. Agar (Bacto) 15.0 g 



4. Lactose (Bacto) 10.0 g 



5. Glucose (Bacto) 1.0 g 



6. NaCl 5.0 g 



7. Andrade Indicator 0.025 g 



Preparation : 



(1) Dissolve 42.0 g. of Bacto Russell 

 Double Sugar Agar (Dehydrated) in 

 1000.0 cc. distilled water by boiling or 

 autoclaving. Avoid excess heat. 



(2) Tube. 



Sterilization: Sterilize in the usual manner. 

 Use: Differentiation of Esch. coli, S. 



paratyphi, S. schotmuelleri, Ebert typhi 



and Ebert dysenteriae. 

 Reference: Digestive Ferments Co. (1925 



p. 12). 



1776. Nichols and Woods' Russel's Double 



Sugar Agar 



Constituents: 



1. Extract agar (3.0%) .". 1000.0 cc. 



2. Lactose (1.0%) 10.0 g. 



3. Glucose (0.1%) 1.0 g. 



4. Phenol red (0.02% soln.) 



(5.0%) 50.0 cc. 



Preparation : 



(1) Prepare extract agar containing 3.0% 

 shred agar. 



(2) Clear (1). 



(3) Add 1.0% lactose 0.1% glucose and 

 5.0% of a 0.02% watery solution of 

 phenol red. 



(4) Correct the reaction to pH 7.2-7.4 hot. 

 Sterilization: Not specified. 



Use: To study fermentation or respiration 



by the typhoid bacillus. 

 Reference : Nichols and Wood (1922 p. 322). 



1777. Bailey and Lacey's Phenol Red Lead 



Acetate Agar 



Constituents: 



1. Tap water 1000.0 cc. 



2. Beef extract (Bacto) 5.0 g. 



3. Peptone (P. D.) 10.0 g. 



4. NaCl (B. & A.) 5.0 g. 



5. Agar 15.0 g. 



6. Lactose 10.0 g. 



7. Glucose 10 g. 



8. Phenol red (0.02%) 50.0 cc. 



9. Lead acetate 0.5 g. 



Preparation : 



(1) Wash the agar in running water. 



(2) Heat in 1000.0 cc. tap water until 

 dissolved. 



(3) Dissolve 2, 3 and 4 in (2). 



(4) Adjust to pH = 7.4. 



(5) Boil for 5 to 8 minutes. 



(6) Readjust the reaction to pH = 7.4. 



(7) Allow to stand and decant the clear 

 supernatant agar. 



(8) Coolto50°C. (to prevent fiocculation) 

 and add 6, 7, 8 and 9. 



(9) Tube. 



Sterilization: Sterilize in the autoclave by 



